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Transplantation Immunology pp 359–371Cite as

Tolerogenic Dendritic Cells and Induction of T Suppressor Cells in Transplant Recipients

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1034))

Abstract

Tolerogenic antigen presenting cells (APC), primarily dendritic cells (DC), are essential to the induction and maintenance of immunologic tolerance in clinical transplantation. They induce the differentiation of CD8+ T suppressor (Ts) and CD4+ T regulatory (Treg) or anergic cells, which prevent transplant rejection maintaining a state of quiescence. Tolerogenic APC express high levels of inhibitory receptors such as Immunoglobulin-like transcript (ILT)3 and 4 which inhibit the effector function of T cells that recognize HLA-peptide complexes on APC. Here, we describe the methods for detection of tolerogenic APC induced by allospecific Ts/Treg cells.

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Author information

Authors and Affiliations

  1. Department of Pathology & Cell Biology, Columbia University, New York, NY, USA

    George Vlad & Nicole Suciu-Foca

Authors
  1. George Vlad
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  2. Nicole Suciu-Foca
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Editor information

Editors and Affiliations

  1. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Andrea A. Zachary

  2. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Mary S. Leffell

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Vlad, G., Suciu-Foca, N. (2013). Tolerogenic Dendritic Cells and Induction of T Suppressor Cells in Transplant Recipients. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_23

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  • DOI: https://doi.org/10.1007/978-1-62703-493-7_23

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-492-0

  • Online ISBN: 978-1-62703-493-7

  • eBook Packages: Springer Protocols

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