Abstract
The enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting antibodies (Abs) and is employed in clinical laboratories to identify Abs against various self-antigens–autoAb development and quantitation. This method relies on specific antigen–Ab interactions where one of the components is immobilized on a solid surface. Using this method, the concentrations of antigens or Ab present in the serum can be quantified with high specificity and accuracy. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
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References
Engvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874
Shulman HM, Sullivan KM (1988) Graft-versus-Host disease: allo- and autoimmunity after bone marrow transplantation. Concepts Immunopathol 6:141–165
Bharat A, Fields RC, Steward N, Trulock EP, Patterson GA et al (2006) CD4+25+ regulatory T cells limit Th1-autoimmunity by inducing IL-10 producing T cells following human lung transplantation. Am J Transplant 6:1799–1808
Milne DS, Gascoigne A, Wilkes J, Sviland L, Ashcroft T et al (1992) The immunohistopathology of obliterative bronchiolitis following lung transplantation. Transplantation 54:748–750
Goers TA, Ramachandran S, Aloush A, Trulock E, Patterson GA et al (2008) De novo production of K-alpha1 tubulin-specific antibodies: role in chronic lung allograft rejection. J Immunol 180:4487–4494
Saini D, Weber J, Ramachandran S, Phelan D, Tiriveedhi V et al (2011) Alloimmunity-induced autoimmunity as a potential mechanism in the pathogenesis of chronic rejection of human lung allografts. J Heart Lung Transplant 30:624–631
Burlingham WJ, Love RB, Jankowska-Gan E, Haynes LD, Xu Q et al (2007) IL-17-dependent cellular immunity to collagen type V predisposes to obliterative bronchiolitis in human lung transplants. J Clin Invest 117:3498–3506
Seetharam AB, Borg BB, Subramanian V, Chapman WC, Crippin JS et al (2011) Temporal association between increased virus-specific Th17 response and spontaneous recovery from recurrent hepatitis C in a liver transplant recipient. Transplantation 92:1364–1370
Borg BB, Seetharam A, Subramanian V, Basha HI, Lisker-Melman M et al (2011) Immune response to extracellular matrix collagen in chronic hepatitis C-induced liver fibrosis. Liver Transpl 17:814–823
Kaczmarek I, Deutsch MA, Kauke T, Beiras-Fernandez A, Schmoeckel M et al (2008) Donor-specific HLA alloantibodies: long-term impact on cardiac allograft vasculopathy and mortality after heart transplant. Exp Clin Transplant 6:229–235
Rose ML, Smith JD (2009) Clinical relevance of complement-fixing antibodies in cardiac transplantation. Hum Immunol 70:605–609
Leong HS, Mahesh BM, Day JR, Smith JD, McCormack AD et al (2008) Vimentin autoantibodies induce platelet activation and formation of platelet-leukocyte conjugates via platelet-activating factor. J Leukoc Biol 83:263–271
Nath DS, Ilias Basha H, Tiriveedhi V, Alur C, Phelan D et al (2010) Characterization of immune responses to cardiac self-antigens myosin and vimentin in human cardiac allograft recipients with antibody-mediated rejection and cardiac allograft vasculopathy. J Heart Lung Transplant 29:1277–1285
Nath DS, Tiriveedhi V, Basha HI, Phelan D, Moazami N et al (2011) A role for antibodies to human leukocyte antigens, collagen-V, and K-alpha1-Tubulin in antibody-mediated rejection and cardiac allograft vasculopathy. Transplantation 91:1036–1043
Acknowledgments
This work was supported by NIH HL056643, HL092514, and the Barnes-Jewish Children’s Foundation to T.M. The authors would like to thank Billie Glasscock for her assistance in submitting this manuscript.
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Sarma, N.J., Tiriveedhi, V., Mohanakumar, T. (2013). Detection of Antibodies to Self-Antigens (K-alpha 1 Tubulin, Collagen I, II, IV, and V, Myosin, and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA). In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_20
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DOI: https://doi.org/10.1007/978-1-62703-493-7_20
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