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Complement-Dependent Cytotoxicity Crossmatch

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Transplantation Immunology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1034))

Abstract

The complement-dependent cytotoxic crossmatch is an informative test that detects alloantibodies in pre- and post-transplant patients, which may dictate clinical management of transplant patients. While challenging to perform, the cytotoxic crossmatch represents the only assay that provides direct evidence for the presence of potentially pathologic (i.e., cytotoxic) alloantibodies. The cytotoxic crossmatch combines patient (recipient) serum and donor cells. If donor-reactive alloantibodies are present in patient serum, these antibodies can bind donor cells. Antibody-antigen complexes, in turn, can activate the complement cascade, leading to complement-mediated cytotoxicity. Two commonly performed cytotoxic crossmatches, using donor lymphocytes as target cells, are described.

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Author information

Authors and Affiliations

  1. Histocompatibility (HLA) Laboratory, Department of Pathology, Massachusetts General Hospital, Boston, MA, USA

    Jeremy Ryan Peña, Donna Fitzpatrick & Susan L. Saidman

Authors
  1. Jeremy Ryan Peña
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  2. Donna Fitzpatrick
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  3. Susan L. Saidman
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Editor information

Editors and Affiliations

  1. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Andrea A. Zachary

  2. , Immunogenetics Laboratory, Johns Hopkins University School of Medic, E. Monument Street 2041, Baltimore, 21205, Maryland, USA

    Mary S. Leffell

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Peña, J.R., Fitzpatrick, D., Saidman, S.L. (2013). Complement-Dependent Cytotoxicity Crossmatch. In: Zachary, A., Leffell, M. (eds) Transplantation Immunology. Methods in Molecular Biology, vol 1034. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-493-7_13

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  • DOI: https://doi.org/10.1007/978-1-62703-493-7_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-492-0

  • Online ISBN: 978-1-62703-493-7

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