Abstract
Natural and synthetic membrane active peptides as well as fragments from membrane proteins interact with membranes. In several cases, such interactions cause the insertion of the peptides to the membrane and their assembly within the lipid bilayer. Here we present spectroscopic approaches utilizing NBD and rhodamine fluorescently labeled peptides to measure peptide–membrane interaction and peptide–peptide interaction within the membrane. The usage of the physical properties of NBD and rhodamine in solution and in membranes provides useful information on the interplay between peptides and lipids.
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Acknowledgments
This work was supported by the Israel Science Foundation, German-Israel Foundation (GIF), Israel Ministry of Health, Benoziyo Center for Neurological Diseases, and European Community Grant No. 278998. Yechiel Shai is the incumbent of the Harold S. and Harriet B. Brady Professorial Chair in Cancer Research.
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Saar-Dover, R., Ashkenazi, A., Shai, Y. (2013). Peptide Interaction with and Insertion into Membranes. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_12
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DOI: https://doi.org/10.1007/978-1-62703-487-6_12
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