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Membrane Biogenesis pp 3–20Cite as

Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1033))

Abstract

Lipid analysis performed by nano-electrospray ionization mass spectrometry is a highly sensitive method for quantification of lipids including all lipid species of a given lipid class. Various instrumental setups are used for quantitative lipid analysis, including different modes of ionization, separation, and detection. Here we describe a work-flow for the rapid and quantitative analysis of lipid species from cellular membranes by direct infusion of lipid extracts to a nano-electrospray ionization triple quadrupole/linear ion trap mass spectrometer.

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Acknowledgment

This work was supported by a grant of the Deutsche Forschungsgemeinschaft (SFB/TRR83). B.B. is an investigator of the CellNetworks Cluster of Excellence (EXC81).

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Authors and Affiliations

  1. Heidelberg University Biochemistry Center (BZH), Heidelberg, Germany

    Cagakan Özbalci, Timo Sachsenheimer & Britta Brügger

Authors
  1. Cagakan Özbalci
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  2. Timo Sachsenheimer
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  3. Britta Brügger
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Editor information

Editors and Affiliations

  1. Interfaculty Institute of Biochemistry, University of Tuebingen, Tuebingen, Germany

    Doron Rapaport

  2. Cell Biology, University of Kaiserslautern, Kaiserslautern, Germany

    Johannes M. Herrmann

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Özbalci, C., Sachsenheimer, T., Brügger, B. (2013). Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_1

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  • DOI: https://doi.org/10.1007/978-1-62703-487-6_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-486-9

  • Online ISBN: 978-1-62703-487-6

  • eBook Packages: Springer Protocols

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