Abstract
Footpad injection is an important route of inoculation in mouse models of disease and immunology. Although commonly used to deliver antigens as a means of eliciting an efficacious immunological response, herein, we describe a protocol for inoculating mice via footpad injection using a hands-free method to deliver infectious material. These procedures allow for efficient delivery of infectious agents in a manner that is safe for both the researcher and animal.
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References
Kawashim Y, Sugimura M, Hwang Y et al (1964) Lymph system in mice. Jpn J Vet Res 12:69–72
Van den Broeck W, Derore A, Simoens P (2006) Anatomy and nomenclature of murine lymph nodes: descriptive study and nomenclatory standardization in BALB/cAnNCrl mice. J Immunol Meth 312(1–2):12–19
Tilney N (1971) Patterns of lymphatic drainage in the adult laboratory rat. J Anat 109(Pt 3):369–383
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Long, K.M., Heise, M. (2013). Safe and Effective Mouse Footpad Inoculation. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_12
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DOI: https://doi.org/10.1007/978-1-62703-481-4_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-480-7
Online ISBN: 978-1-62703-481-4
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