Abstract
Innate immune responses often result in the activation and modulation of T lymphocyte function. Analysis of T lymphocytes in mouse models of innate immunity can allow understanding of the links between the innate and adaptive immune systems. Other T lymphocyte populations display innate-like functions. Isolation of T cells and evaluation of their surface proteins can provide data on T cell activation, as can an analysis of T cell proliferation. Further insight may be obtained by examining cytokine production via intracellular cytokine staining or ELISPOT to determine T cell function. This chapter describes methods for T cell isolation, measurement of surface protein expression, T cell proliferation, intracellular cytokine staining, and ELISPOT.
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Barker, B.R. (2013). Measuring T Cell Function in Innate Immune Models. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_10
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DOI: https://doi.org/10.1007/978-1-62703-481-4_10
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-480-7
Online ISBN: 978-1-62703-481-4
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