Skip to main content
SpringerLink
Log in

Size-Dependent Study of Pulmonary Responses to Nano-sized Iron and Copper Oxide Nanoparticles

  • Protocol
  • First Online:
Oxidative Stress and Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1028))

Abstract

The application of nanotechnology in various fields has resulted in a tremendous increase in the synthesis of variety of engineered nanoparticles (NPs). These applications are possible only due to the small size and large surface area of the NPs which imparts them unique properties. Inorganic oxide NPs as iron and copper oxide NPs are widely used in several biomedical and synthetic applications. The beneficial aspects of these NPs are concurrently associated with several drastic and deleterious effects as well. Size of the NPs plays a critical role in systemic clearance from the body. Initial studies have confirmed inflammatory responses in mice associated with non-biodegradable oxide NPs. The associated oxidative stress varied from mild effects to reactive oxygen species generation which can potentiate DNA damage or even induced carcinogenesis. Copper oxide NPs, in particular, induced acute toxicity and inflict neutrophil infiltration. This chapter focuses on the applicability of various in vivo techniques for studying the effect of these NPs, especially on the pulmonary system. These in vivo techniques would certainly provide a better understanding and insight into the mechanistic pathways by which these NPs interact with various organ systems in human body.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Dvir T, Timko BP, Kohane DS, Langer R (2011) Nanotechnological strategies for engineering complex tissues. Nat Nanotech 6:13–22

    Article  CAS  Google Scholar 

  2. Dobrovolskaia MA, McNeil SE (2007) Immunological properties of engineered nanomaterials. Nat Nanotech 2:469–478

    Article  CAS  Google Scholar 

  3. Teow Y, Asharani PV, Hande MP, Valiyaveettil S (2011) Health impact and safety of engineered nanomaterials. Chem Commun 47:7025–7038

    Article  CAS  Google Scholar 

  4. Maynard AD, Aitken RJ, Butz T, Colvin V, Donaldson K, Oberdörster G, Philbert MA, Ryan J, Seaton A, Stone V, Tinkle SS, Tran L, Walker NJ, Warheit DB (2006) Safe handling of nanotechnology. Nature 444:267–269

    Article  CAS  Google Scholar 

  5. Cho K, Wang X, Nie S, Chen Z, Shin DM (2008) Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res 14:1310–1316

    Article  CAS  Google Scholar 

  6. Ren G, Hu D, Cheng EW, Vargas-Reus MA, Reip P, Allaker RP (2009) Characterisation of copper oxide nanoparticles for antimicrobial applications. Int J Antimicrob Agents 33:587–590

    Article  CAS  Google Scholar 

  7. Eastman JA, Choi SUS, Li S, Yu W, Thompson LJ (2001) Anomalously increased effective thermal conductivities of ethylene glycol-based nanofluids containing copper nanoparticles. Appl Phys Lett 78:718–720

    Article  CAS  Google Scholar 

  8. Guo L, Huang Q, Li X, Yang S (2001) Iron nanoparticles: synthesis and applications in surface enhanced Raman scattering and electrocatalysis. Phys Chem Chem Phys 3:1661–1665

    Article  CAS  Google Scholar 

  9. Gupta AK, Gupta M (2005) Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 26:3995–4021

    Article  CAS  Google Scholar 

  10. Ito A, Shinkai M, Honda H, Kobayashi T (2005) Medical application of functionalized magnetic nanoparticles. J Biosci Bioeng 100:1–11

    Article  CAS  Google Scholar 

  11. Karlsson HL, Gustafsson J, Cronholma P, Möller L (2009) Size-dependent toxicity of metal oxide particles—a comparison between nano- and micrometer size. Toxicol Lett 188:112–118

    Article  CAS  Google Scholar 

  12. Oberdorster G, Stone V, Donaldson K (2007) Toxicology of nanoparticles: a historical perspective. Nanotoxicology 1:2–25

    Article  CAS  Google Scholar 

  13. Weissleder R, Stark DD, Engelstad BL, Bacon BR, Compton CC, White DL, Jacobs P, Lewis J (1989) Superparamagnetic iron oxide: pharmacokinetics and toxicity. AJR Am J Roentgenol 152:167–173

    Article  CAS  Google Scholar 

  14. Yokohira M, Hashimoto N, Yamakawa K, Suzuki S, Saoo K, Kuno T, Imaida K (2009) Lung carcinogenic bioassay of CuO and TiO2 nanoparticles with intratracheal instillation using F344 rats. J Toxicol Pathol 22:71–78

    Article  CAS  Google Scholar 

  15. Nel A, Xia T, Mädler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–627

    Article  CAS  Google Scholar 

  16. Singh N, Jenkins GJS, Asadi R, Doak SH (2010) Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION). Nano Rev 1:5358–5372

    Google Scholar 

  17. Warheit DB, Webb TR, Reed KL, Frerichs S, Sayes CM (2007) Pulmonary toxicity study in rats with three forms of ultrafine-TiO2 particles: differential responses related to surface properties. Toxicology 230:90–104

    Article  CAS  Google Scholar 

  18. Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci 88:412–419

    Article  CAS  Google Scholar 

  19. Xia T, Kovochich M, Brant J, Hotze M, Sempf J, Oberley T, Sioutas C, Yeh JI, Wiesner MR, Nel AE (2006) Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett 6:1794–1807

    Article  CAS  Google Scholar 

  20. Cho W-S, Duffin R, Poland CA, Howie SEM, MacNee W, Bradley M, Megson IL, Donaldson K (2010) Metal oxide nanoparticles induce unique inflammatory footprints in the lung: important implications for nanoparticle testing. Environ Health Perspect 118:1699–1706

    Article  CAS  Google Scholar 

  21. Fahmy B, Cormier SA (2009) Copper oxide nanoparticles induce oxidative stress and cytotoxicity in airway epithelial cells. Toxicol In Vitro 23:1365–1371

    Article  CAS  Google Scholar 

  22. Guo B, Zebda R, Drake SJ, Sayes CM (2009) Synergistic effect of co-exposure to carbon black and Fe2O3 nanoparticles on oxidative stress in cultured lung epithelial cells. Part Fibre Toxicol 6:4–16

    Article  Google Scholar 

  23. Ying E, Hwang H-M (2010) In vitro evaluation of the cytotoxicity of iron oxide nanoparticles with different coatings and different sizes in A3 human T lymphocytes. Sci Total Environ 408:4475–4481

    Article  CAS  Google Scholar 

  24. Olive PL, Banáth JP (2006) The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 1:23–29

    Article  CAS  Google Scholar 

  25. Brian JD, Knudson DE, Sorokin SP, Davis MA (1976) Pulmonary distribution of particles given by intratracheal instillation or by aerosol inhalation. Environ Res 11:13–33

    Article  Google Scholar 

Download references

Acknowledgement

This work was supported by Electronics Materials Research Institute (eMRI), Department of Physics, Northeastern University.

Author information

Authors and Affiliations

  1. Department of Physics, Northeastern University and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA

    Rajiv Kumar & Dattatri K. Nagesha

Authors
  1. Rajiv Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dattatri K. Nagesha
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Florida, n/a, Gainsville, 32611, Florida, USA

    Donald Armstrong

  2. , Pharmaceutical Research Institute, Albany College of Pharmacy, Discovery Lane 1, Rensselaer, 12144, USA

    Dhruba J Bharali

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, New York

About this protocol

Cite this protocol

Kumar, R., Nagesha, D.K. (2013). Size-Dependent Study of Pulmonary Responses to Nano-sized Iron and Copper Oxide Nanoparticles. In: Armstrong, D., Bharali, D. (eds) Oxidative Stress and Nanotechnology. Methods in Molecular Biology, vol 1028. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-475-3_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-475-3_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-474-6

  • Online ISBN: 978-1-62703-475-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation