Abstract
Nanotechnology involves the creation and manipulation of materials at nanoscale levels to create products that exhibit novel properties. Engineered nanomaterials either metals (like carbon and silver) or metal oxides (like zinc oxide, magnesium oxide, and titanium oxide) induce toxicity and oxidative stress by generating free radicals. Various in vitro and in vivo models are available to estimate the oxidative stress induced by the nanoparticles. In this chapter, we describe the methods for the estimation of oxidative stress markers like reactive oxygen species (ROS), DNA damage estimation, and lipid peroxidation products; total antioxidant capacity (TAC) was mentioned.
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References
Griendling KK, FitzGerald GA (2003) Oxidative stress and cardiovascular injury: part I: basic mechanisms and in vivo monitoring of ROS. Circulation 108:1912–1916
Hoet PH, Bruske-Hohlfeld I, Salata OV (2004) Nanoparticles—known and unknown health risks. J Nanobiotechnol 1:12–14
Thomas K, Sayre P (2005) Research strategies for safety evaluation of nanomaterials, part I: evaluating the human health implications of exposure to nanoscale materials. Toxicol Sci 2:316–321
Brown DM, Stone V, Findlay P et al (2000) Increased inflammation and intracellular calcium caused by ultrafine carbon black is independent of transition metals or other soluble components. Occupat Environ Med 57:685–691
Derfus AM, Chan WCW, Bhatia SN et al (2004) Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 4(1):11–18
Foley S, Crowley C, Smaihi M et al (2002) Cellular localisation of a water-soluble fullerene derivative. Biochem Biophys Res Commun 294(1):116–119
Oberdorster G, Maynard A, Yang H et al (2005) Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy 1. Part Fibre Toxicol 2(1):8–14
Reddy ARN, Rao MV, Krishan DR et al (2012) Pulmonary toxicity assessment of multi-wall carbon nanotubes in rats following intratracheal instillation. Environ Toxicol 27(4):211–219
Anreddy RNR, Devarakonda RK, Vurimindi H et al (2011) In vitro toxicity of multi wall carbon nanoparticles on Hep G 32 liver cell lines. Lat Am J Pharm 30(1):177–80
Lam C, James TJ, McClusky R, Hunter LR (2003) Pulmonary toxicity of carbon nanotubes in mice 7 and 90 days after intratracheal instillation. Toxicology 72(S-1):44–48
Hussain SM, Hess KL, Gearhart JM, Geiss KT (2005) In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro 19:975–983
Anreddy RNR, Yellu NR, Devarakonda RK et al (2010) Multi wall carbon nanoparticles induce oxidative stress and cytotoxicity in human embryonic kidney (HEK293) cells. Toxicology 272:11–16
Reddy ARN, Rao MV, Krishan DR et al (2011) Evaluation of oxidative stress and antioxidant status in rats following exposure of carbon nanotubes. Regul Toxicol Pharmacol 59:251–257
Reddy ARN, Rao MV, Krishan DR et al (2011) Induction of oxidative stress and cytotoxicity by carbon nanomaterials is dependent on physical properties. Toxicol Ind Health 27:3–10
Oberdorster E (2004) Manufactured nanomaterials (fullerenes, C60) induce oxidative stress in the brain of juvenile largemouth bass. Environ Health Perspect 112:1058–1062
Reddy ARN, Rao MV, Krishan DR et al (2011) Induction of oxidative stress and cytotoxicity by carbon nanomaterials is dependent on physical properties. Toxicol Ind Health 27:3–10
Wang H, Joseph JA (1999) Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 5–6:612–616
Keston AS, Brandt R (1965) The fluorometric analysis of ultramicro quantities of hydrogen peroxide. Anal Biochem 11:1–5
Patel PR, Bevan RJ, Mistry N et al (2007) Evidence of oligonucleotides containing 8-hydroxy-2′-deoxyguanosine in human urine. J Free Radic Biol Med 42:552–558
Shen J, Deininger P, Hunt JD, Zhao H (2007) 8-Hydroxy-2′-deoxyguanosine (8-OH-dG) as a potential survival biomarker in patients with non-small cell lung cancer. Cancer 109(3):574–580
Dubuisson ML, de Wergifosse B, Trouet A, Baguet F et al (2000) Antioxidative properties of natural coelenterazine and synthetic methyl coelenterazine in rat hepatocytes subjected to tert-butyl hydroperoxide-induced oxidative stress. Biochem Pharmacol 60:471–478
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Deleve LD, Kaplowitz N (1991) Glutathione metabolism and its role in hepatotoxicity. Pharmacol Ther 52:287–305
Shaik IH, Mehvar R (2006) Rapid determination of reduced and oxidized glutathione levels using a new thiol-masking reagent and the enzymatic recycling method: application to the rat liver and bile samples. Anal Bioanal Chem 385:105–113
Cerutti P, Trump B (1991) Inflammation and oxidative stress in carcinogenesis. Proc Cancer Cell 3:1–7
Trachootham D, Lu W, Ogasawara MA et al (2008) Redox regulation of cell survival. Antioxid Redox Signal 10:1343–1374
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Anreddy, R.N.R., Yellu, N.R., Devarakonda, K.R. (2013). Oxidative Biomarkers to Assess the Nanoparticle-Induced Oxidative Stress. In: Armstrong, D., Bharali, D. (eds) Oxidative Stress and Nanotechnology. Methods in Molecular Biology, vol 1028. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-475-3_13
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DOI: https://doi.org/10.1007/978-1-62703-475-3_13
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