Abstract
This chapter discusses the preparation and subsequent profiling capabilities of gold nanoparticle-oligonucleotide conjugates for multiple melanoma mRNA targets. We will outline the attachment of DNA hairpins modified with a thiol for facile attachment to gold nanoparticle surfaces through gold-sulfur bond formation. Furthermore, the ability of these conjugates to detect and distinguish phenotypic variations utilizing several melanoma cell lines and the nonmalignant cell line, HEp-2, will be investigated using flow cytometry and RT-PCR analytical techniques. The behavior of the housekeeping probe β-actin will also be investigated as a control.
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Stone, J.W., Harry, R., Hendley, O., Wright, D.W. (2013). Gold Nanoparticle–Oligonucleotide Conjugates for the Profiling of Malignant Melanoma Phenotypes. In: Rosenthal, S., Wright, D. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology, vol 1026. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-468-5_9
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DOI: https://doi.org/10.1007/978-1-62703-468-5_9
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Publisher Name: Humana Press, Totowa, NJ
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