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In Vitro O-Antigen Ligase Assay

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Glycosyltransferases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1022))

Abstract

WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the proximal end of the undecaprenyl-diphosphate (Und-PP)-O-antigen with a terminal sugar of the lipid A-core oligosaccharide (OS). This is a critical step in lipopolysaccharide synthesis. We describe here an assay to perform the ligation reaction in vitro utilizing native substrates.

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Acknowledgments

This work was supported by a grant from the Canadian Institutes of Health Research to M.A.V., who also holds a Canada Research Chair in Infectious Diseases and Microbial Pathogenesis.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Centre for Human Immunology, University of Western Ontario, London, ON, Canada

    Xiang Ruan

  2. Centre for Infection and Immunity, Queen’s University Belfast, Belfast, UK

    Miguel A. Valvano

Authors
  1. Xiang Ruan
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  2. Miguel A. Valvano
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Editor information

Editors and Affiliations

  1. Department of Medicine, Queen's University, Kingston, Ontario, Canada

    Inka Brockhausen

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Ruan, X., Valvano, M.A. (2013). In Vitro O-Antigen Ligase Assay. In: Brockhausen, I. (eds) Glycosyltransferases. Methods in Molecular Biology, vol 1022. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-465-4_15

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  • DOI: https://doi.org/10.1007/978-1-62703-465-4_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-464-7

  • Online ISBN: 978-1-62703-465-4

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