Abstract
Functionalization is a key element in biodetection technologies such as micro/nano-mechanical sensors. Since assay sensitivity and stability drastically depends on a proper bioreceptor immobilization, the sensing surface must be first chemically modified with uniform, well-packed, and robust layers. Here, we describe three functionalization protocols that we developed for the surface modification with amino, aldehyde, and carboxyl groups of micro/nano-mechanical biosensors.
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Acknowledgments
The authors wish to thank the financial support of Regione Piemonte (Converging Technologies 2007—NAMATECH grant, Poli di Innovazione POR-FESR 2007–2013—CANESTRO grant, and Piattaforme Innovative POR-FESR 2007–2013—ITACA and SAFE-FOOD CONTROL grants) and Italian Ministry of Research and Education (FIRB 2010—NEWTON grant, Progetti Bandiera 2011—NANOMAX grant).
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Frascella, F., Ricciardi, C. (2013). Functionalization Protocols of Silicon Micro/Nano-mechanical Biosensors. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_8
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DOI: https://doi.org/10.1007/978-1-62703-462-3_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-461-6
Online ISBN: 978-1-62703-462-3
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