Abstract
Polymeric coatings, usually referred as tridimensional chemistries, provide homogenous surface derivatization methods presenting a high reactive group concentration and resulting in an increased binding capacity of targets. Furthermore, they act as linkers distributing the bound probe also in the axial position, thus causing a faster reaction with the target involved in biomolecular recognition and can be engineered to custom tailor their properties for specific applications.
Most approaches which aim at attaching polymers to a surface use a system where the polymer carries an “anchor” group either as an end group or in a side chain. This anchor group can reacts with appropriate sites at the substrate surface, thus yielding surface-attached monolayers of polymer molecules (termed “grafting to”). Another technique is to carry out a polymerization reaction in the presence of a substrate onto which monomers had been attached leading to the so called “grafting from” approach.
In this chapter, protocols to functionalize glass and silicon surfaces by “grafting to” as well as by “grafting-from” approach are shown using copolymers made of N,N-dimethylacrylamide (DMA) or Glycidyl methacrylate (GMA) as the polymer backbone, N-acryloyloxysuccinimide (NAS) as reactive group, and 3-(trimethoxysilyl)propyl methacrylate (MAPS) or 3-mercaptopropyl trimethoxy silane (MPS) as anchoring groups.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Krenkler KP, Laible R, Hamann K (1976) Polyreaktionen an Pigmentoberflächen. VII. Mitteilung: Reaktionen von Polymeren mit endständigen Chlorsilangruppen an Siliciumdioxidoberflächen. Angew Makromol Chem 53:101–123
Tsubokawa N et al (1990) Grafting onto carbon black: reaction of functional groups on carbon black with ACYL chloride-capped polymers. J Macromol Sci 27:445–457
Tsubokawa N, Kuroda A, Sone Y (1989) Grafting onto carbon black by the reaction of reactive carbon black having epoxide groups with several polymers. J Polym Sci A27:1701–1712
Dimitrenko AV et al (1990) Polymer—inorganic selective adsorbents for gas chromatography produced by graft polymerization. J Chromatogr A 520:21–31
Hashimoto K et al (1982) Graft copolymerization of glass fiber and its application. J Macromol Sci 18:173–190
Klein J et al (1993) Lubrication forces between surfaces bearing polymer brushes. Macromolecules 26:5552–5560
Yoshikawa C et al (2006) Protein repellency of well-defined, concentrated poly(2-hydroxyethyl methacrylate) brushes by the size-exclusion effect. Macromolecules 39:2284–2290
Pirri G et al (2006) Microarray glass slides coated with block copolymer brushes obtained by reversible addition chain-transfer polymerization. Anal Chem 78:3118–3124
Tsubokawa N et al (1989) Graft polymerization of acrylamide from ultra silica particles by use of a redox system consisting of ceric ion and reducing groups on the surface. Polym J 21:475–481
Prucker O, Rühe J (1998) Synthesis of poly(styrene) monolayers attached to high surface area silica gels through self-assembled monolayers of azo initiators. Macromolecules 31:592–601
Prucker O, Rühe J (1998) Mechanism of radical chain polymerizations initiated by azo compounds covalently bound to the surface of spherical particles. Macromolecules 31:602–613
Di Carlo G et al (2012) Synthesis and conformational characterization of functional di-block copolymer brusche for microarray technology. Appl Surf Sci 258:3750–3756
Acknowledgments
Financial support from the Italian Ministry of University and Research PRIN 2008 (2008JWKYXB_005), Italian Institute of Technology (project SEED “IPG-CHIP”), and Fondazione Cariplo (SpinBioMed, grant # 2008–2330) are gratefully acknowledged.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Sola, L., Cretich, M., Damin, F., Chiari, M. (2013). Surface Modifications by Polymers for Biomolecule Conjugation. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_7
Download citation
DOI: https://doi.org/10.1007/978-1-62703-462-3_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-461-6
Online ISBN: 978-1-62703-462-3
eBook Packages: Springer Protocols