Abstract
We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inorganic nanoparticle with the enhanced DNA binding properties that originate from the high surface DNA density. In particular, the hybrid nanostructures exhibit orders of magnitude higher binding constants than regular DNA strands. This chapter presents a detailed protocol for the preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.
Key words
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- CPG:
-
Controlled pore glass
- DLS:
-
Dynamic light scattering
- DMF:
-
N,N-Dimethylformamide
- DNA-b-PS:
-
Block copolymer of DNA and polystyrene
- FAM:
-
Fluorescein
- FRET:
-
Förster/Fluorescent Resonance Energy Transfer
- MNP:
-
Magnetic nanoparticles
- MNP@PS@DNA:
-
DNA-b-polystyrene assemblies loaded with magnetic nanoparticles
- PBS:
-
Phosphate buffer saline
- PS:
-
Polystyrene
- PS-MNP:
-
Polystyrene modified magnetic nanoparticles
- PS@DNA:
-
Self assembly of DNA-b-polystyrene
- PTFE:
-
Polytetrafluoroethylene
- TEM:
-
Transmission electron microscope
- THF:
-
Tetrahydrofuran
References
Bardhan R, Lal S, Joshi A, Halas NJ (2011) Theranostic nanoshells: from probe design to imaging and treatment of cancer. Acc Chem Res 44:936–946
Della Rocca J, Liu D, Lin W (2011) Nanoscale metal-organic frameworks for biomedical imaging and drug delivery. Acc Chem Res 44:957–968
Xie J, Liu G, Eden HS, Ai H, Chen X (2011) Surface-engineered magnetic nanoparticle platforms for cancer imaging and therapy. Acc Chem Res 44:883–892
Erathodiyil N, Ying JY (2011) Functionalization of inorganic nanoparticles for bioimaging applications. Acc Chem Res 44:925–935
Yoo D, Lee J-H, Shin T-H, Cheon J (2011) Theranostic magnetic nanoparticles. Acc Chem Res 44:863–874
Kievit FM, Zhang M (2011) Surface engineering of iron oxide nanoparticles for targeted cancer therapy. Acc Chem Res 44:853–862
Chen X-J, Sanchez-Gaytan BL, Qian Z, Park S-J (2012) Noble metal nanoparticles in DNA detection and delivery. Wiley Interdiscip Rev Nanomed Nanobiotechnol 4:273–290
Rosi NL, Mirkin CA (2005) Nanostructures in biodiagnostics. Chem Rev 105:1547–1562
Chen X-J, Sanchez-Gaytan BL, Hayik SEN, Fryd M, Wayland BB, Park S-J (2010) Self-assembled hybrid structures of DNA block-copolymers and nanoparticles with enhanced DNA binding properties. Small 6:2256–2260
Li Z, Zhang Y, Fullhart P, Mirkin CA (2004) Reversible and chemically programmable micelle assembly with DNA block-copolymer amphiphiles. Nano Lett 4:1055–1058
Alemdaroglu FE, Ding K, Berger R, Herrmann A (2006) DNA-templated synthesis in three dimensions: introducing a micellar scaffold for organic reactions. Angew Chem Int Ed 45:4206–4210
Jeong JH, Park TG (2001) Novel Polymer-DNA hybrid polymeric micelles composed of hydrophobic Poly(D, L-lactic-co-glycolic Acid) and hydrophilic oligonucleotides. Bioconjug Chem 12:917–923
Chien M-P, Rush AM, Thompson MP, Gianneschi NC (2010) Programmable shape-shifting micelles. Angew Chem Int Ed 49:5076–5080
Northwestern University. oligonucleotide properties calculator used to calculate extinction coefficient of DNA. http://www.basic.northwestern.edu/biotools/OligoCalc.html
Sun S, Zeng H (2002) Size-controlled synthesis of magnetite nanoparticles. J Am Chem Soc 124:8204–8205
Clegg RM, David M.J.L.a.J.E.D. (1992) Fluorescence resonance energy transfer and nucleic acids. Methods Enzymol, vol. 211, Academic, p 353–388
The information is available at the Glen Research website. Glen Research. http://www.glenresearch.com/
Kuno M, Lee JK, Dabbousi BO, Mikulec FV, Bawendi MG (1997) The band edge luminescence of surface modified CdSe nanocrystallites: probing the luminescing state. J Chem Phys 106:9869–9882
Zimmermann J, Kwak M, Musser AJ, Herrmann A (2011) Amphiphilic DNA block copolymers: nucleic acid-polymer hybrid materials for diagnostics and biomedicine. Methods Mol Biol 751:239–266
Acknowledgments
This work was supported by the NSF Career Award (DMR-087646) and NSF-IGERT Fellowship (DGE-0221664).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Chen, XJ., Hickey, R.J., Park, SJ. (2013). Preparation and Characterization of DNA Block Copolymer Assemblies Loaded with Nanoparticles. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_16
Download citation
DOI: https://doi.org/10.1007/978-1-62703-462-3_16
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-461-6
Online ISBN: 978-1-62703-462-3
eBook Packages: Springer Protocols