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Nanomaterial Interfaces in Biology pp 157–166Cite as

Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Adsorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1025))

Abstract

The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-­depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-­competition kinetics from binary solution.

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Acknowledgments

This work was supported by National Institute of Health grant PHS 5R01HL069965. The authors appreciate the support from the Departments of Materials Science and Engineering and Bioengineering, The Pennsylvania State University.

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Authors and Affiliations

  1. Department of Optometry and Vision Science, Seoul National University and Technology, Seoul, South Korea

    Hyeran Noh

  2. Division of Physics, Rajamangala University of Technology, Thanyaburi, Pathum Thani, Thailand

    Naris Barnthip

  3. Department of Chemistry, Ravenshaw University, Cuttack, Orissa, India

    Purnendu Parhi

  4. Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA, USA

    Erwin A. Vogler

  5. Department of Bioengineering, The Pennsylvania State University, University Park, PA, USA

    Erwin A. Vogler

Authors
  1. Hyeran Noh
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  2. Naris Barnthip
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  3. Purnendu Parhi
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  4. Erwin A. Vogler
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Editor information

Editors and Affiliations

  1. Dipto di Ingegneria Meccanica, Università degli Studi di Brescia, Brescia, Italy

    Paolo Bergese

  2. Dept of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    Kimberly Hamad-Schifferli

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Noh, H., Barnthip, N., Parhi, P., Vogler, E.A. (2013). Electrophoretic Implementation of the Solution-Depletion Method for Measuring Protein Adsorption, Adsorption Kinetics, and Adsorption Competition Among Multiple Proteins in Solution. In: Bergese, P., Hamad-Schifferli, K. (eds) Nanomaterial Interfaces in Biology. Methods in Molecular Biology, vol 1025. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-462-3_12

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  • DOI: https://doi.org/10.1007/978-1-62703-462-3_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-461-6

  • Online ISBN: 978-1-62703-462-3

  • eBook Packages: Springer Protocols

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