Abstract
Real-time and noninvasive imaging of intracellular second messengers in mammalian cells, while preserving their in vivo phenotype, requires biosensors of exquisite constitution. Here we provide the methodology for utilizing the single wavelength cGMP-biosensor δ-FlincG in aortic vascular smooth muscle cells.
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Acknowledgments
The authors would like to thank Drs. Carolyn Sawyer, Sharon Cawley, and Lydia Nausch for their help in perfecting this technique. Support was provided by NIH grants HL68991 (W.R.D.) and T323 HL07944 (K.F.H.), and the Totman Trust for Biomedical Research.
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Held, K.F., Dostmann, W.R. (2013). Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells. In: Krieg, T., Lukowski, R. (eds) Guanylate Cyclase and Cyclic GMP. Methods in Molecular Biology, vol 1020. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-459-3_8
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DOI: https://doi.org/10.1007/978-1-62703-459-3_8
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