Abstract
Radioimmunoassay is an established method to determine the amount of a specific substance in a given cell or tissue sample. Commercially available RIA or Elisa are very cost intensive. Here, we describe the generation of radioactive cGMP tracer and the quantification of cGMP. Although working with radioactive material requires experience and care, this method is very sensitive and rather cheap, once it is established.
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References
Gilman AG (1972) A protein binding assay for adenosine 3’:5’-cyclic monophosphate. Proc Natl Acad Sci USA 67:305–312
Steiner AL, Wehmann RE, Parker CW, Kipnis DM (1972) Radioimmunoassay for the measurement of cyclic nucleotides. Adv Cyclic Nucleotide Res 2:51–61
Brooker G, Harper JF, Terasaki WL, Moylan RD (1979) Radioimmunoassay of cyclic AMP and cyclic GMP. Adv Cyclic Nucleotide Res 10:1–33
Friebe A, Müllershausen F, Smolenski A, Walter U, Schultz G, Koesling D (1998) YC-1 potentiates NO- and CO-induced cGMP effects in human platelets. Mol Pharmacol 54:962–967
Müllershausen F, Russwurm M, Koesling D, Friebe A (2004) In vivo reconstitution of the negative feedback in nitric oxide/cGMP signaling: role of phosphodiesterase type 5 phosphorylation. Mol Biol Cell 15:4023–4030
Müllershausen F, Lange A, Mergia E, Friebe A, Koesling D (2006) Desensitization of NO/cGMP signaling in smooth muscle: blood vessels versus airways. Mol Pharmacol 69:1969–1974
Friebe A, Mergia E, Dangel O, Lange A, Koesling D (2007) Fatal gastrointestinal obstruction and hypertension in mice lacking nitric oxide-sensitive guanylyl cyclase. Proc Natl Acad Sci USA 104(18):7699–7704
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Jäger, R., Groneberg, D., Lies, B., Bettaga, N., Kümmel, M., Friebe, A. (2013). Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues. In: Krieg, T., Lukowski, R. (eds) Guanylate Cyclase and Cyclic GMP. Methods in Molecular Biology, vol 1020. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-459-3_4
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DOI: https://doi.org/10.1007/978-1-62703-459-3_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-458-6
Online ISBN: 978-1-62703-459-3
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