Abstract
Oral cancer diagnosis can be greatly facilitated by early diagnosis in order to improve the 50 % 5-year mortality that has not changed much over the last years. Saliva is an easily accessible medium that has been shown to contain microvesicles (exosomes) that enclose microRNAs. We have previously demonstrated that the majority of salivary microRNAs are within exosomes. MicroRNAs have been implicated in oral cancer and the use of salivary exosomal microRNAs holds the promise of identification of diagnostic and prognostic markers.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lambert R et al (2011) Epidemiology of cancer from the oral cavity and oropharynx. Eur J Gastroenterol Hepatol 23(8):633–641
Scully C (2011) Oral cancer aetiopathogenesis; past, present and future aspects. Med Oral Patol Oral Cir Bucal 16(3):e306–e311
Pinkston JA, Cole P (1999) Incidence rates of salivary gland tumors: results from a population-based study. Otolaryngol Head Neck Surg 120(6):834–840
Ryu OH et al (2006) Identification of parotid salivary biomarkers in Sjogren’s syndrome by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and two-dimensional difference gel electrophoresis. Rheumatology (Oxford) 45(9):1077–1086
Juusola J, Ballantyne J (2007) mRNA profiling for body fluid identification by multiplex quantitative RT-PCR. J Forensic Sci 52(6): 1252–1262
Park NJ et al (2009) Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection. Clin Cancer Res 15(17):5473–5477
Michael A et al (2010) Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis 16(1):34–38
Chua JH, Armugam A, Jeyaseelan K (2009) MicroRNAs: biogenesis, function and applications. Curr Opin Mol Ther 11(2):189–199
Liu CJ et al (2012) Exploiting salivary miR-31 as a clinical biomarker of oral squamous cell carcinoma. Head Neck 34(2):219–224
Gallo A et al (2012) The majority of microRNAs detectable in serum and saliva is concentrated in exosomes. PLoS One 7(3):e30679
Acknowledgements
This research was supported by the Intramural Research Program of the National Institute of Dental and Craniofacial Research.
We would like to thank Dr. Gabor Illei for his intellectual contributions and Lolita Bebris, Tammy Yokum, and Dr. Margaret Grissius for the optimization of the saliva collection.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Gallo, A., Alevizos, I. (2013). Isolation of Circulating MicroRNA in Saliva. In: Kosaka, N. (eds) Circulating MicroRNAs. Methods in Molecular Biology, vol 1024. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-453-1_14
Download citation
DOI: https://doi.org/10.1007/978-1-62703-453-1_14
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-452-4
Online ISBN: 978-1-62703-453-1
eBook Packages: Springer Protocols