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Circulating MicroRNAs pp 147–155Cite as

Direct Serum Assay for MicroRNA in Cancer Patients

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1024))

Abstract

MicroRNAs (miRs) are small noncoding RNAs which can be detected in body fluids as well as cells and tissues. miRs play important roles in various activities of cancer cells. The miRs in bloods called circulating miRs (cmiRs) are potential biomarkers for detecting and monitoring cancer progression. There are reports on the cmiR research which utilizes various primers, reagents, and instruments. Here, we introduce our protocols for RNA extraction and RT-qPCR for cmiRs as well as our novel RT-qPCR directly in serum assay (RT-qPCR-DS) where RT is directly performed in serum without the need for RNA extraction. Results from the two protocols are analyzed and compared. RT-qPCR-DS is logistically simpler and more sensitive in assessing cmiR in breast cancer patients than isolating RNA before RT-qPCR. This approach may be very useful as a diagnostic tool.

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Author information

Authors and Affiliations

  1. Department of Molecular Oncology, John Wayne Cancer Institute at Saint John’s Health Center, Santa Monica, CA, USA

    Sota Asaga & Dave S. B. Hoon

Authors
  1. Sota Asaga
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  2. Dave S. B. Hoon
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Editor information

Editors and Affiliations

  1. , Div. of Molecular and Cellular Medicine, National Cancer Center Research Institut, Tsukiji 5-chome, Chuo-ku 1-1, Tokyo, 104-0045, Japan

    Nobuyoshi Kosaka

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Asaga, S., Hoon, D.S.B. (2013). Direct Serum Assay for MicroRNA in Cancer Patients. In: Kosaka, N. (eds) Circulating MicroRNAs. Methods in Molecular Biology, vol 1024. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-453-1_11

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  • DOI: https://doi.org/10.1007/978-1-62703-453-1_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-452-4

  • Online ISBN: 978-1-62703-453-1

  • eBook Packages: Springer Protocols

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