Skip to main content

Cryosectioning

  • Protocol
  • First Online:
Neural Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1018))

Abstract

Cryosectioning, the sectioning of frozen specimens, has been an important histological tool for more than a century and continues to be extensively utilized today. However, the ability to produce high-quality sections is often a difficult process requiring extensive patience and experience. In this chapter, we have detailed an effective method for the embedding, mounting, and sectioning of frozen tissues, as well as have provided suggestions in producing high-quality sections.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Gal AA, Cagle PT (2005) The 100-year ­anniversary of the description of the frozen ­section procedure. JAMA 294(24):3135–3137. doi:294/24/3135 [pii] 10.1001/jama.294.24.3135

    Article  PubMed  CAS  Google Scholar 

  2. Dahlin DC (1980) Seventy-five years’ experience with frozen sections at the Mayo Clinic. Mayo Clinic Proc 55(11):721–723

    CAS  Google Scholar 

  3. Wilson LB (1905) A method for the rapid preparation of fresh tissues for the microscope. JAMA 45:1

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Son, A.I., Sokolowski, K., Zhou, R. (2013). Cryosectioning. In: Zhou, R., Mei, L. (eds) Neural Development. Methods in Molecular Biology, vol 1018. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-444-9_28

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-444-9_28

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-443-2

  • Online ISBN: 978-1-62703-444-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics