Abstract
Cryosectioning, the sectioning of frozen specimens, has been an important histological tool for more than a century and continues to be extensively utilized today. However, the ability to produce high-quality sections is often a difficult process requiring extensive patience and experience. In this chapter, we have detailed an effective method for the embedding, mounting, and sectioning of frozen tissues, as well as have provided suggestions in producing high-quality sections.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Gal AA, Cagle PT (2005) The 100-year anniversary of the description of the frozen section procedure. JAMA 294(24):3135–3137. doi:294/24/3135 [pii] 10.1001/jama.294.24.3135
Dahlin DC (1980) Seventy-five years’ experience with frozen sections at the Mayo Clinic. Mayo Clinic Proc 55(11):721–723
Wilson LB (1905) A method for the rapid preparation of fresh tissues for the microscope. JAMA 45:1
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Son, A.I., Sokolowski, K., Zhou, R. (2013). Cryosectioning. In: Zhou, R., Mei, L. (eds) Neural Development. Methods in Molecular Biology, vol 1018. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-444-9_28
Download citation
DOI: https://doi.org/10.1007/978-1-62703-444-9_28
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-443-2
Online ISBN: 978-1-62703-444-9
eBook Packages: Springer Protocols