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Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro

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Neural Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1018))

Abstract

Neural progenitor cells (NPCs) or neural stem cells are important tools for investigating central nervous system (CNS) development. NPCs can be used in therapeutic strategies and for characterizing differentiation mechanisms. Here, we describe methods for isolating and culturing embryonic NPCs.

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References

  1. Reynolds BA, Tetzlaff W, Weiss S (1992) A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J Neurosci 12:4565–4574

    PubMed  CAS  Google Scholar 

  2. Gotz M, Huttner WB (2005) The cell biology of neurogenesis. Nat Rev Mol Cell Biol 6:777–788

    Article  PubMed  Google Scholar 

  3. Temple S (2001) The development of neural stem cells. Nature 414:112–117

    Article  PubMed  CAS  Google Scholar 

  4. Kriegstein A, Alvarez-Buylla A (2009) The glial nature of embryonic and adult neural stem cells. Annu Rev Neurosci 32:149–184

    Article  PubMed  CAS  Google Scholar 

  5. Shimazaki T (2003) Biology and clinical application of neural stem cells. Horm Res 60(Suppl 3):1–9

    Article  PubMed  CAS  Google Scholar 

  6. Liste I, Garcia-Garcia E, Martinez-Serrano A (2004) The generation of dopaminergic neurons by human neural stem cells is enhanced by Bcl-XL, both in vitro and in vivo. J Neurosci 24:10786–10795

    Article  PubMed  CAS  Google Scholar 

  7. Sher F et al (2008) Differentiation of neural stem cells into oligodendrocytes: involvement of the polycomb group protein Ezh2. Stem Cells 26:2875–2883

    Article  PubMed  CAS  Google Scholar 

  8. Tate MC et al (2002) Fibronectin promotes survival and migration of primary neural stem cells transplanted into the traumatically injured mouse brain. Cell Transplant 11:283–295

    PubMed  Google Scholar 

  9. Tate CC et al (2009) Laminin and fibronectin scaffolds enhance neural stem cell transplantation into the injured brain. J Tissue Eng Regen Med 3:208–217

    Article  PubMed  CAS  Google Scholar 

  10. Bez A et al (2003) Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization. Brain Res 993:18–29

    Article  PubMed  CAS  Google Scholar 

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Kim, MY., Moon, BS., Choi, KY. (2013). Isolation and Maintenance of Cortical Neural Progenitor Cells In Vitro. In: Zhou, R., Mei, L. (eds) Neural Development. Methods in Molecular Biology, vol 1018. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-444-9_1

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  • DOI: https://doi.org/10.1007/978-1-62703-444-9_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-443-2

  • Online ISBN: 978-1-62703-444-9

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