Abstract
Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein–protein interactions. Investigating interactions of proteins containing “sticky” polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.
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Acknowledgments
This work was supported by grants from the National Health and Medical Research Council of Australia (Project Grant 1002732 to C.S.; Principle Research Fellowship 508043 to J.G.), Australian Research Council Future Fellowship 120100086 to C.S., and MS McLeod Foundation postgraduate scholarship to M.H.T.
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Mattiske, T.R., Tan, M.H., Gécz, J., Shoubridge, C. (2013). Challenges of “Sticky” Co-immunoprecipitation: Polyalanine Tract Protein–Protein Interactions. In: Hatters, D., Hannan, A. (eds) Tandem Repeats in Genes, Proteins, and Disease. Methods in Molecular Biology, vol 1017. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-438-8_9
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DOI: https://doi.org/10.1007/978-1-62703-438-8_9
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