Skip to main content
SpringerLink
Log in
Book cover

Tandem Repeats in Genes, Proteins, and Disease pp 95–104Cite as

Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1017))

Abstract

Fragile X syndrome, the leading inherited cause of mental retardation and autism spectrum disorders worldwide, is caused by a tandem repeat expansion in the FMR1 (fragile X mental retardation 1) gene. It presents with a distinct behavioral phenotype which overlaps significantly with that of autism. Emerging evidence suggests that tandem repeat polymorphisms (TRPs) might also play a key role in modulating disease susceptibility for a range of common polygenic disorders, including the broader autism spectrum of disorders (ASD) and other forms of psychiatric illness such as schizophrenia, depression, and bipolar disorder [1]. In order to understand how TRPs and associated gene mutations mediate pathogenesis, various mouse models have been generated. A crucial step in such functional genomics is high-quality behavioral and cognitive phenotyping. This chapter presents a basic behavioral battery for standardized tests for assaying social phenotypes in mouse models of brain disorders, with a focus on aggression.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Hannan AJ (2010) Tandem repeat polymorphisms: modulators of disease susceptibility and candidates for ‘missing heritability’. Trends Genet 26(2):59–65. doi:10.1016/j.tig.2009.11.008

    Article  PubMed  CAS  Google Scholar 

  2. Hannan AJ (2012) Tandem repeat polymorphisms: genetic plasticity, neural diversity and disease. Landes Bioscience and Springer Science+Business Media, New York. Springer series: Advances in Experimental Medicine and Biology 769:1–208.

    Google Scholar 

  3. Crawley JN, Schleidt WM, Contrera JF (1975) Does social environment decrease propensity to fight in male mice? Behav Biol 15:73–83

    Article  PubMed  CAS  Google Scholar 

  4. Miczek KA, O’Donnell JM (1978) Intruder-evoked aggression in isolated and nonisolated mice: effects of psychomotor stimulants and L-dopa. Psychopharmacology (Berl) 57(1):47–55

    Article  CAS  Google Scholar 

  5. Brain P, Benton D (1979) The interpretation of physiological correlates of differential housing in laboratory rats. Life Sci 24(2):99–115

    Article  PubMed  CAS  Google Scholar 

  6. Miczek KA, Maxson SC, Fish EW, Faccidomo S (2001) Aggressive behavioral phenotypes in mice. Behav Brain Res 125(1–2):167–181

    Article  PubMed  CAS  Google Scholar 

  7. Miczek KA, Fish EW, De Bold JF (2003) Neurosteroids, GABAA receptors, and escalated aggressive behavior. Horm Behav 44(3):242–257

    Article  PubMed  CAS  Google Scholar 

  8. Miczek KA, Fish EW, De Bold JF, De Almeida RM (2002) Social and neural determinants of aggressive behavior: pharmacotherapeutic targets at serotonin, dopamine and gamma-aminobutyric acid systems. Psychopharmacology 163(3–4):434–458. doi:10.1007/s00213-002-1139-6

    Article  PubMed  CAS  Google Scholar 

  9. Chavez B, Chavez-Brown M, Rey JA (2006) Role of risperidone in children with autism spectrum disorder. Ann Pharmacother 40(5):909–916. doi:10.1345/aph.1G389

    Article  PubMed  CAS  Google Scholar 

  10. Erickson C, Stigler K, Wink L, Mullett J, Kohn A, Posey D, McDougle C (2011) A prospective open-label study of aripiprazole in fragile X syndrome. Psychopharmacology 216(1):85–90. doi:10.1007/s00213-011-2194-7

    Article  PubMed  CAS  Google Scholar 

  11. Miczek KA, Faccidomo S, De Almeida RMM, Bannai M, Fish EW, Debold JF (2004) Escalated aggressive behavior: new pharmacotherapeutic approaches and opportunities. Ann N Y Acad Sci 1036(1):336–355. doi:10.1196/annals.1330.021

    Article  PubMed  CAS  Google Scholar 

  12. Velez L, Sokoloff G, Miczek KA, Palmer AA, Dulawa SC (2010) Differences in aggressive behavior and DNA copy number variants between BALB/cJ and BALB/cByJ substrains. Behav Genet 40(2):201–210. doi:10.1007/s10519-009-9325-5

    Article  PubMed  Google Scholar 

  13. Cleves M, Gould W, Gutierrez R, Marchenko Y (2008) An introduction to survival analysis using STATA, 2nd edn. Stata Press, College Station, TX

    Google Scholar 

  14. Yang M, Silverman JL, Crawley JN (2011) Automated three-chambered social approach task for mice. Current protocols in neuroscience, Chapter 8:Unit 8.26. doi:10.1002/0471142301.ns0826s56

  15. Yang M, Crawley JN (2009) Simple behavioral assessment of mouse olfaction. Current protocols in neuroscience, Chapter 8:Unit 8.24. doi:10.1002/0471142301.ns0824s48

  16. Komada M, Takao K, Miyakawa T (2008) Elevated plus maze for mice. J Vis Exp (22):e1088. doi:10.3791/1088

  17. Kudryavtseva NN, Bondar NP, Avgustinovich DF (2004) Effects of repeated experience of aggression on the aggressive motivation and development of anxiety in male mice. Neurosci Behav Physiol 34(7):721–730

    Article  PubMed  CAS  Google Scholar 

  18. Bouet V, Lecrux B, Tran G, Freret T (2011) Effect of pre- versus post-weaning environmental disturbances on social behaviour in mice. Neurosci Lett 488(2):221–224. doi:10.1016/j.neulet.2010.11.033

    Article  PubMed  CAS  Google Scholar 

  19. Kercmar J, Budefeld T, Grgurevic N, Tobet SA, Majdic G (2011) Adolescent social isolation changes social recognition in adult mice. Behav Brain Res 216(2):647–651. doi:10.1016/j.bbr.2010.09.007

    Article  PubMed  Google Scholar 

  20. Koike H, Ibi D, Mizoguchi H, Nagai T, Nitta A, Takuma K, Nabeshima T, Yoneda Y, Yamada K (2009) Behavioral abnormality and pharmacologic response in social isolation-reared mice. Behav Brain Res 202(1):114–121. doi:10.1016/j.bbr.2009.03.028

    Article  PubMed  CAS  Google Scholar 

  21. Rawleigh JM, Kemble ED, Ostrem J (1993) Differential effects of prior dominance or subordination experience on conspecific odor preferences in mice. Physiol Behav 54:35–39

    Article  PubMed  CAS  Google Scholar 

  22. Guillot PV, Chapouthier G (1996) Intermale aggression and dark/light preference in ten inbred mouse strains. Behav Brain Res 77(1–2):211–213

    Article  PubMed  CAS  Google Scholar 

  23. Dow HC, Kreibich AS, Kaercher KA, Sankoorikal GM, Pauley ED, Lohoff FW, Ferraro TN, Li H, Brodkin ES (2011) Genetic dissection of intermale aggressive behavior in BALB/cJ and A/J mice. Genes Brain Behav 10(1):57–68. doi:10.1111/j.1601-183X.2010.00640.x

    Article  PubMed  CAS  Google Scholar 

  24. Chabout J, Serreau P, Ey E, Bellier L, Aubin T, Bourgeron T, Granon S (2012) Adult male mice emit context-specific ultrasonic vocalizations that are modulated by prior isolation or group rearing environment. PLoS One 7(1):e29401. doi:10.1371/journal.pone.0029401

    Article  PubMed  CAS  Google Scholar 

  25. Lumley LA, Sipos ML, Charles RC, Charles RF, Meyerhoff JL (1999) Social stress effects on territorial marking and ultrasonic vocalizations in mice. Physiol Behav 67(5):769–775

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was funded by grants to A.J.H. from the National Health and Medical Research Council (APP1034785 and APP1047674). A.J.H. is an Australian Research Council Future Fellow (FT3).

Author information

Authors and Affiliations

  1. Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre, University of Melbourne, Parkville, VIC, Australia

    Emma L. Burrows & Anthony J. Hannan

Authors
  1. Emma L. Burrows
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anthony J. Hannan
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. , Dept. of Biochemistry and Molecular Biol, The University of Melbourne, Flemington Road 30, Parkville, 3010, Victoria, Australia

    Danny M. Hatters

  2. Florey Neuroscience Institutes, and Mental Health, The University of Melbourne, Royal Parade, Parkville, 3010, Victoria, Australia

    Anthony J. Hannan

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, New York

About this protocol

Cite this protocol

Burrows, E.L., Hannan, A.J. (2013). Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders. In: Hatters, D., Hannan, A. (eds) Tandem Repeats in Genes, Proteins, and Disease. Methods in Molecular Biology, vol 1017. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-438-8_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-438-8_7

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-437-1

  • Online ISBN: 978-1-62703-438-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation