Abstract
The Illumina GoldenGate Assay is a technique that is widely used in molecular genetics to analyze up to thousands of single nucleotide polymorphism (SNPs) simultaneously, providing data of very high quality in a fast and efficient manner. This technique allows the user to optimize the number of genetic loci to be interrogated in a way that best suits their research goals. Here are described in detail all the steps to be followed in the process of genotyping a custom panel, from panel design through data analysis.
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Designing custom GoldenGate genotyping assay. Technical note (from www.illumina.com)
GoldenGate® assay workflow. Technical note (from www.illumina.com)
Analyzing GoldenGate genotyping data. Technical note (from www.illumina.com)
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González-Neira, A. (2013). The GoldenGate Genotyping Assay: Custom Design, Processing, and Data Analysis. In: Innocenti, F., van Schaik, R. (eds) Pharmacogenomics. Methods in Molecular Biology, vol 1015. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-435-7_9
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DOI: https://doi.org/10.1007/978-1-62703-435-7_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-434-0
Online ISBN: 978-1-62703-435-7
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