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The Myc Gene pp 135–144Cite as

Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1012))

Abstract

As a global transcription factor, Myc regulates both protein-coding genes and noncoding microRNA genes. Myc-activated or repressed miRNAs are involved in various pathways to affect tumorigenesis, mediate apoptosis, proliferation, angiogenesis, metastasis, and metabolism downstream of Myc. Functional characterization of miRNAs in the Myc network requires the accurate detection and quantification of miRNA expression levels. Here, we describe two widely used methodologies to determine miRNA expression, including miRNA real-time PCR and miRNA northern analysis.

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Acknowledgment

L.H. acknowledges the support of an RO1 and an R21 grant from NCI (R01 CA139067, 1R21CA175560-01); a joint grant from National Science Foundation (NSF, CBET-1015026); a new faculty award from CIRM (RN2-00923-1), a research grant from TRDRP (21RT-0133) and a research scholar award from American Cancer Society (ACS, 123339-RSG-12-265-01-RMC).

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Authors and Affiliations

  1. Department of Molecular & Cell Biology, University of California at Berkeley, Berkeley, CA, USA

    Rui Song, Nicole Sponer & Lin He

Authors
  1. Rui Song
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  2. Nicole Sponer
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  3. Lin He
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Editor information

Editors and Affiliations

  1. Fundació Vall d'Hebron Institute of Onco, Barcelona, Spain

    Laura Soucek

  2. Department of Biochemistry Sanger Building, University of Cambridge, Cambridge, United Kingdom

    Nicole M. Sodir

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Song, R., Sponer, N., He, L. (2013). Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression. In: Soucek, L., Sodir, N. (eds) The Myc Gene. Methods in Molecular Biology, vol 1012. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-429-6_10

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  • DOI: https://doi.org/10.1007/978-1-62703-429-6_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-428-9

  • Online ISBN: 978-1-62703-429-6

  • eBook Packages: Springer Protocols

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