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Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice

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Chemokines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1013))

Abstract

Over the past 25 years, genetically engineered mouse models have become an integral and invaluable research tool to develop our understanding of mammalian physiology and pathology. This unit describes methods for generating transgenic mice, focusing on reporter animals relevant to chemokine receptor and ligand expression. Specifically, we describe the use of bacterial artificial chromosome (BAC) engineering and embryonic stem cell manipulation to generate “knock in” and transgenic mice.

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Author information

Authors and Affiliations

  1. The Rayne Institute, University College London, London, UK

    Simon Yona

  2. Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel

    Ki-Wook Kim, Rebecca Haffner & Steffen Jung

Authors
  1. Simon Yona
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  2. Ki-Wook Kim
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  3. Rebecca Haffner
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  4. Steffen Jung
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Editor information

Editors and Affiliations

  1. Department of Biology, South Texas Center for Emerging Infectious Diseases, University of Texas at San Antonio, One UTSA Circle, San Antonio, 78249, Texas, USA

    Astrid E. Cardona

  2. , Department of Neurology, Baylor College of Medicine, One Baylor Plaza, Houston, 77030, Texas, USA

    Eroboghene E. Ubogu

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Yona, S., Kim, KW., Haffner, R., Jung, S. (2013). Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice. In: Cardona, A., Ubogu, E. (eds) Chemokines. Methods in Molecular Biology, vol 1013. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-426-5_8

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  • DOI: https://doi.org/10.1007/978-1-62703-426-5_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-425-8

  • Online ISBN: 978-1-62703-426-5

  • eBook Packages: Springer Protocols

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