Abstract
Neural stem cells (NSCs) represent a limited population of progenitor cells in the central nervous system that sustain their self-renewal and multipotency from early development to adulthood. Recent evidence suggests that chemokine receptors are constitutively expressed by NSCs and are directly involved in stem cell biology. As cell surface receptors, chemokine receptors also provide an important avenue to enrich these cells and further identify the potential molecular pathways required to maintain their biological functions. Here, I describe in vitro methods that have been widely applied to sort, culture, maintain, and differentiate NSCs.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Zlotnik A, Yoshie O (2000) Chemokines: a new classification system and their role in immunity. Immunity 12:121–127
Fredriksson R, Lagerström MC, Lundin LG, Schiöth HB (2003) The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol Pharmacol 63:1256–1272
Kawasawa Y, McKenzie LM, Hill DP, Bono H, Yanagisawa M et al (2003) G-protein-coupled receptor genes in the FANTOM2 database. Genome Res 13:1466–1477
DeVries ME, Kelvin AA, Xu L, Ran L, Robinson J, Kelvin DJ (2006) Defining the origins and evolution of the chemokine/chemokine receptor system. J Immunol 176:401–415
Rot A, von Andrian UH (2004) Chemokines in innate and adaptive host defense: basic chemokinese grammar for immune cells. Annu Rev Immunol 22:891–928
Li M, Ransohoff RM (2008) Multiple roles of chemokine CXCL12 in the central nervous system: a migration from immunology to neurobiology. Prog Neurobiol 84:116–131
Li M, Ransohoff RM (2009) The roles of chemokine CXCL12 in embryonic and brain tumor angiogenesis. Semin Cancer Biol 19:111–115
Kokovay E, Goderie S, Wang Y, Lotz S, Lin G, Sun Y et al (2010) Adult SVZ lineage cells home to and leave the vascular niche via differential responses to SDF1/CXCR4 signaling. Cell Stem Cell 7:163–173
Corti S, Locatelli F, Papadimitriou D, Donadoni C, Del BR, Fortunato F et al (2005) Multipotentiality, homing properties, and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells. FASEB J 19:1860–1872
Dziembowska M, Tham TN, Lau P, Vitry S, Lazarini F, Dubois-Dalcq M (2005) A role for CXCR4 signaling in survival and migration of neural and oligodendrocyte precursors. Glia 50:258–269
Gong X, He X, Qi L, Zuo H, Xie Z (2006) Stromal cell derived factor-1 acutely promotes neural progenitor cell proliferation in vitro by a mechanism involving the ERK1/2 and PI-3K signal pathways. Cell Biol Int 30:466–471
Peng H, Huang Y, Rose J, Erichsen D, Herek S, Fujii N et al (2004) Stromal cell-derived factor 1-mediated CXCR4 signaling in rat and human cortical neural progenitor cells. J Neurosci Res 76:35–50
Tran PB, Ren D, Veldhouse TJ, Miller RJ (2004) Chemokine receptors are expressed widely by embryonic and adult neural progenitor cells. J Neurosci Res 76:20–34
Wu Y, Peng H, Cui M, Whitney NP, Huang Y, Zheng JC (2009) CXCL12 increases human neural progenitor cell proliferation through Akt-1/FOXO3a signaling pathway. J Neurochem 109:1157–1167
Li M, Chang CJ, Lathia JD, Wang L, Pacenta HL, Cotleur A, Ransohoff RM (2011) Chemokine receptor CXCR4 signaling modulates the growth factor-induced cell cycle of self-renewing and multipotent neural progenitor cells. Glia 59:108–118
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Li, M. (2013). Chemokine Receptors and Neural Stem Cells. In: Cardona, A., Ubogu, E. (eds) Chemokines. Methods in Molecular Biology, vol 1013. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-426-5_4
Download citation
DOI: https://doi.org/10.1007/978-1-62703-426-5_4
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-425-8
Online ISBN: 978-1-62703-426-5
eBook Packages: Springer Protocols