Abstract
Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.
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Acknowledgments
This work was supported by grants from Instituto de Salud Carlos III (PI11/02239, FIS PI070537, FIS PI080970) and Mutua Madrileña Automovilista (20174/004), Redes temáticas de Investigación Cooperativa en Salud (RD06/0014/1015), and Fundación para la Investigación Sanitaria de Castilla-La Mancha (FISCAM PI2008-08, PI2008-28, PI2008-52).
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de la Cuesta, F., Alvarez-Llamas, G., Maroto, A.S., Barderas, M.G., Vivanco, F. (2013). Laser Microdissection and Saturation Labeling DIGE Method for the Analysis of Human Arteries. In: Vivanco, F. (eds) Vascular Proteomics. Methods in Molecular Biology, vol 1000. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-405-0_2
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DOI: https://doi.org/10.1007/978-1-62703-405-0_2
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