Abstract
Protein glycosylation plays an important role in multiple cell functions, and aberrations of protein glycosylation are associated with various malignancies including cancer. In this chapter, we provide a detailed protocol for MALDI MS analysis of permethylated N-glycans extracted from human serum proteins. The protocol includes procedures for N-glycan purification and in-solution permethylation, structural elucidation of permethylated N-glycans by MALDI-QIT-TOF MS, and construction of indices to quantify levels of certain types of glycosylation, such as fucosylation, which may serve as a potential disease biomarker.
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Acknowledgment
We thank Dr. Andy Lo for helpful comments during writing of the manuscript.
We acknowledge support of this work from the National Cancer Institute under grant 1 R01 CA154455 01 (DML) and the SPORE program grant 1 P50CA130810 (DML, DMS, MTR) and from the National Institutes of Health under grant R01 GM49500 (DML) and grant K23 DK082097 (MAA).
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Lin, Z., Lubman, D.M. (2013). Permethylated N-Glycan Analysis with Mass Spectrometry. In: Matthiesen, R. (eds) Mass Spectrometry Data Analysis in Proteomics. Methods in Molecular Biology, vol 1007. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-392-3_12
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DOI: https://doi.org/10.1007/978-1-62703-392-3_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-391-6
Online ISBN: 978-1-62703-392-3
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