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Microsatellite Fragment Analysis Using the ABI Prism ® 377 DNA Sequencer

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Microsatellites

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1006))

Abstract

The ABI Prism ® 377 DNA Sequencer is used for a variety of microsatellite-based research. The platform provides researchers with a cost-effective means for high-throughput genotyping, which can be further optimized by multiplexing microsatellite loci or by using a tail-labeling approach to screen large sets of markers. The goals of this chapter are to present a protocol for performing microsatellite-based analyses on the ABI 377 and to provide researchers with information on how to troubleshoot common issues associated with running the ABI 377 sequencers.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA

    Mark A. Renshaw

  2. Center for Biosystematics and Biodiversity, Texas A&M University, College Station, TX, USA

    Melissa Giresi

  3. Azco Biotech Inc., San Diego, CA, USA

    J. Orville Adams

Authors
  1. Mark A. Renshaw
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  2. Melissa Giresi
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  3. J. Orville Adams
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Editor information

Editors and Affiliations

  1. , Department of Plant, Soil,, Southern Illinois University, Carbondale, Lincoln Drive 1205, Carbondale, 62901, Illinois, USA

    Stella K. Kantartzi

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Renshaw, M.A., Giresi, M., Adams, J.O. (2013). Microsatellite Fragment Analysis Using the ABI Prism ® 377 DNA Sequencer. In: Kantartzi, S. (eds) Microsatellites. Methods in Molecular Biology, vol 1006. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-389-3_13

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  • DOI: https://doi.org/10.1007/978-1-62703-389-3_13

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-388-6

  • Online ISBN: 978-1-62703-389-3

  • eBook Packages: Springer Protocols

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