Abstract
Degenerative aortic stenosis is the most common worldwide cause of valve replacement. While it shares certain risk factors with coronary artery disease, it is not delayed or reversed by reducing exposure to risk factors (e.g., therapies that lower lipids). Therefore, it is necessary to better understand its pathophysiology for preventive measures to be taken. In order to identify proteins that are involved in AS, we designed a simple method for protein extraction, digestion, labeling, identification, and differential protein expression analysis of both normal and stenotic aortic valve to identify biomarkers of this pathology, which may facilitate early diagnosis and treatment.
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Acknowledgments
This work was supported by grants from the Instituto de Salud Carlos III (FIS PI070537, PI11/02239, PI11/01401), Fondos Feder, Redes temáticas de Investigación Cooperativa en Salud (RD06/0014/1015), and Fundación para la Investigación Sanitaria de Castilla-La Mancha (FISCAM PI2008-08, PI2008-28, PI2008-52).
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Alonso-Orgaz, S., Martin-Rojas, T., Calvo, E., López, J.A., Vivanco, F., Barderas, M.G. (2013). Differential Protein Expression Analysis of Degenerative Aortic Stenosis by iTRAQ Labeling. In: Vivanco, F. (eds) Heart Proteomics. Methods in Molecular Biology, vol 1005. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-386-2_9
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DOI: https://doi.org/10.1007/978-1-62703-386-2_9
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-385-5
Online ISBN: 978-1-62703-386-2
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