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Necrosis pp 67–73Cite as

Analysis of Pyroptosis in Bacterial Infection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1004))

Abstract

Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells.

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Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA

    Lia Danelishvili & Luiz E. Bermudez

  2. Department of Microbiology, College of Science, Oregon State University, Corvallis, OR, USA

    Luiz E. Bermudez

  3. Program of Molecular and Cell Biology, Oregon State University, Corvallis, OR, USA

    Luiz E. Bermudez

Authors
  1. Lia Danelishvili
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  2. Luiz E. Bermudez
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Editor information

Editors and Affiliations

  1. Department of Biology, Boston University, Boston, MA, USA

    Kimberly McCall

  2. Brookline, MA, USA

    Charles Klein

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Danelishvili, L., Bermudez, L.E. (2013). Analysis of Pyroptosis in Bacterial Infection. In: McCall, K., Klein, C. (eds) Necrosis. Methods in Molecular Biology, vol 1004. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-383-1_6

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  • DOI: https://doi.org/10.1007/978-1-62703-383-1_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-382-4

  • Online ISBN: 978-1-62703-383-1

  • eBook Packages: Springer Protocols

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