Abstract
Mammalian species have evolved a large and diverse number of odorant receptors (ORs). These proteins comprise the largest family of G-protein-coupled receptors (GPCRs) known, amounting to ∼1,000 different receptors in the rodent. From the perspective of olfactory coding, the availability of such a vast number of chemosensory receptors poses several fascinating questions; in addition, such a large repertoire provides an attractive biological model to study ligand–receptor interactions. The limited functional expression of these receptors in heterologous systems, however, has greatly hampered attempts to deorphanize them. We have employed a successful approach that combines electrophysiological and imaging techniques to analyze the response profiles of single sensory neurons. Our approach has enabled us to characterize the “odor space” of a population of native aldehyde receptors and the molecular range of a genetically engineered receptor, OR-I7.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Malnic B, Hirono J, Sato T, Buck LB (1999) Combinatorial receptor codes for odors. Cell 96:713–723
Mombaerts P (2004) Genes and ligands for odorant, vomeronasal and taste receptors. Nat Rev Neurosci 5:263–278
Reisert J, Restrepo D (2009) Molecular tuning of odorant receptors and its implication for odor signal processing. Chem Senses 34:535–545
Touhara K (2007) Deorphanizing vertebrate olfactory receptors: recent advances in odorant-response assays. Neurochem Int 51: 132–139
Matsunami H, Mainland JD, Dey S (2009) Trafficking of mammalian chemosensory receptors by receptor-transporting proteins. Ann N Y Acad Sci 1170:153–156
Araneda RC, Peterlin Z, Zhang X, Chesler A, Firestein S (2004) A pharmacological profile of the aldehyde receptor repertoire in rat olfactory epithelium. J Physiol 555: 743–756
Kajiya K, Inaki K, Tanaka M, Haga T, Kataoka H, Touhara K (2001) Molecular bases of odor discrimination: reconstitution of olfactory receptors that recognize overlapping sets of odorants. J Neurosci 21:6018–6025
Kaluza JF, Breer H (2000) Responsiveness of olfactory neurons to distinct aliphatic aldehydes. J Exp Biol 203(Pt 5):927–933
Araneda RC, Kini AD, Firestein S (2000) The molecular receptive range of an odorant receptor. Nat Neurosci 3:1248–1255
Yuste R, Lanni F, Konnerth A (2000) Imaging neurons: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Buck L, Axel R (1991) A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell 65:175–187
McClintock TS, Sammeta N (2003) Trafficking prerogatives of olfactory receptors. Neuroreport 14:1547–1552
Saito H, Kubota M, Roberts RW, Chi Q, Matsunami H (2004) RTP family members induce functional expression of mammalian odorant receptors. Cell 119:679–691
Peterlin Z, Li Y, Sun G, Shah R, Firestein S, Ryan K (2008) The importance of odorant conformation to the binding and activation of a representative olfactory receptor. Chem Biol 15:1317–1327
Acknowledgment
The authors would like to thank Xinmin Zhang for his helpful input in developing and optimizing these procedures.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Smith, R.S., Peterlin, Z., Araneda, R.C. (2013). Pharmacology of Mammalian Olfactory Receptors. In: Crasto, C. (eds) Olfactory Receptors. Methods in Molecular Biology, vol 1003. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-377-0_15
Download citation
DOI: https://doi.org/10.1007/978-1-62703-377-0_15
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-376-3
Online ISBN: 978-1-62703-377-0
eBook Packages: Springer Protocols