Abstract
Human pluripotent stem cells (hPSCs), including embryonic stem (ES) and induced pluripotent stem (iPS) cells, have been historically cultured in media containing xenogeneic animal components. As hPSC-derived cells and tissues are being developed for human therapies, the application of culture systems to reduce potential immunoreactivity and improve reproducibility becomes increasingly vital. Methods for directly adapting hPSCs to a commercially available culture system free of nonhuman proteins (xeno-free) are described in this chapter.
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Acknowledgments
The author extends many thanks to Marian Piekarczyk for her significant contributions to this work and critical review of the chapter. Thanks to David Piper for his encouragement and critical review of the chapter. Thanks to Justin Wetter and Jun Wang for their assistance with hPSC culture and flow cytometry. Lastly, thanks to my family for their love and support.
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Sampsell-Barron, T. (2013). Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_8
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DOI: https://doi.org/10.1007/978-1-62703-363-3_8
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