Abstract
Activity of cytochrome p450 (CYP450) enzyme is used to measure the ability of hepatocytes to metabolize pharmaceutical compounds. When determining functionality of hepatocytes, the cells may be induced in order to determine metabolic activity after drug induction. Hepatocytes, whether in suspension or plated, are used in the pharmaceutical industry as a surrogate to assess in vivo drug metabolism. Within this chapter, isolation of hepatocytes from whole-liver tissue and subsequent preparation for CYP450 istotype 3A4 activity is discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Miltry RR, Hugher RD, Dhawan A (2002) Progress in human hepatocytes: Isolation, culture & cryopreservation. Cell Dev Biol 13:463–467
Cho J-J, Joseph B, Sappal BS et al (2004) Analysis of the functional integrity of cryoperserved human liver cells including Âxenografting in immunodeficient mice to address suitability for clinical applications. Liver Int 24:361–370
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Meheux, S.O. (2013). Cell Isolation Through Whole-Liver Perfusion and Preparation of Hepatocytes for Cytochrome P450 Analysis. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_5
Download citation
DOI: https://doi.org/10.1007/978-1-62703-363-3_5
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-362-6
Online ISBN: 978-1-62703-363-3
eBook Packages: Springer Protocols