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Genotypic and Phenotypic Analysis of In Vivo Tissue Regeneration in an Animal Model

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  • First Online:
Organ Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1001))

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Abstract

Determining the in vivo response to cellular therapies is important in evaluating the effectiveness of regenerative medicine therapies. Such treatment modalities leverage the treated individual’s ability to elicit the body’s innate healing response to repair/regenerate damaged tissues or organs. Detailed within this chapter is the process of evaluating the host tissue response to a candidate cell therapy through analysis of key transcript and protein targets.

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References

  1. Bonventre JV (2003) Dedifferentiation and proliferation of surviving epithelial cells in acute renal failure. J Am Soc Nephrol 14:S55–S61

    Article  PubMed  Google Scholar 

  2. Guo JK, Cantley LG (2010) Cellular maintenance and repair of the kidney. Ann Rev Physiol 72:357–376

    Article  CAS  Google Scholar 

  3. Lin F, Moran A, Igarashi P (2005) Intrarenal cells, not bone marrow-derived cells, are the major source for regeneration in postischemic kidney. J Clin Invest 115:1756–1764

    Article  PubMed  CAS  Google Scholar 

  4. Kelley R et al (2010a) Bioactive renal cells augment kidney function in a rodent model of chronic kidney disease. International Society for Cell Therapy conference. www.tengion.com/news/documents/Kelley%202010%20ISCT%20podium%20FINAL.pdf

  5. Kelley R et al (2010) A tubular cell-enriched subpopulation of primary renal cells improves survival and augments kidney function in a rodent model of chronic kidney disease. Am J Physiol Renal Physiol 299:F1026–F1039

    Article  PubMed  CAS  Google Scholar 

  6. Presnell SC et al (2010) Isolation, characterization, and expansion (ICE) methods for defined primary renal cell populations from rodent, canine, and human normal and diseased kidneys. Tissue Eng Part C Methods 17:261–273

    Article  PubMed  Google Scholar 

  7. Sagrinati C et al (2006) Isolation and characterization of multipotent progenitor cells from the Bowman’s capsule of adult human kidneys. J Am Soc Nephrol 17:2443–2456

    Article  PubMed  CAS  Google Scholar 

  8. Sagrinati C et al (2008) Stem-cell approaches for kidney repair: choosing the right cells. Trends Mol Med 14:277–285

    Article  PubMed  CAS  Google Scholar 

  9. van den Boom V et al (2007) UTF1 is a chromatin-associated protein involved in ES cell differentiation. J Cell Biol 178:913–924

    Article  PubMed  Google Scholar 

  10. Chambers I, Tomlinson SR (2009) The transcriptional foundation of pluripotency. Development 136:2311–2322

    Article  PubMed  CAS  Google Scholar 

  11. Shen MM (2007) Nodal signaling: developmental roles and regulation. Development 134:1023–1034

    Article  PubMed  CAS  Google Scholar 

  12. Tabibzadeh S, Hemmati-Brivanlou A (2006) Lefty at the crossroads of “stemness” and differentiative events. Stem Cells 24:1998–2006

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Tengion Inc., Winston-Salem, NC, USA

    Christopher W. Genheimer

Authors
  1. Christopher W. Genheimer
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Editors and Affiliations

  1. Tengion, Inc, Westpoint Blvd. 3929, Winston-Salem, 27103, North Carolina, USA

    Joydeep Basu

  2. Zen Bio, Inc., E Hwy 54 3200, Research Triangle Park, 27709, North Carolina, USA

    John W. Ludlow

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© 2013 Springer Science+Business Media New York

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Genheimer, C.W. (2013). Genotypic and Phenotypic Analysis of In Vivo Tissue Regeneration in an Animal Model. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_28

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  • DOI: https://doi.org/10.1007/978-1-62703-363-3_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-362-6

  • Online ISBN: 978-1-62703-363-3

  • eBook Packages: Springer Protocols

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