Abstract
A tissue-engineered product needs to be properly characterized in order to be used in vivo. Many methods can be used to characterize a scaffold during creation of a tissue-engineered product. This chapter looks at the mechanical (tensile testing) and biological characterization (cell viability and proliferation) of a polyglycolic acid-based scaffold used to tissue engineer a Neo-Urinary Conduitâ„¢. Such methods are more broadly applicable to characterization of other neo-organ product candidates.
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References
Standard guide for characterization and testing of biomaterial scaffolds used in tissue-engineered medical products. ASTM Standard: F2150-07
Barbucci R (2002) Integrated biomaterials science. Kluwer,  
Basu J, Jayo MJ, Ilagan RM et al (2012) Regeneration of native-like neo-urinary tissue from nonbladder cell sources. Tissue Eng Part A 18:1025–34
Ratner BD, Hoffman AS, Schoen FJ et al (1996) Biomaterials science: an introduction to materials in medicine. Elsevier Science,  
Maltaris T, Kaya H, Hoffmann I et al (2006) Comparison of xenografting in SCID mice and LIVE/DEAD assay as a predictor of the developmental potential of cryopreserved ovarian tissue. In Vivo 20:11–16
Soman G, Yang X, Jiang H et al (2009) MTS dye based colorimetric CTLL-2 cell proliferation assay for product release and stability monitoring of Interleukin-15: Assay qualification, standardization and statistical analysis. J Immunol Methods 348:83–94
CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay (MTS) - http://www.promega.com/products/cell-health-assays/cell-viability-assays/celltiter-96-aqueous-non_radioactive-cell-Âproliferation-assay-_mts_/. Accessed on January 22, 2013
Standard test method for tensile properties of plastics 2010. ASTM Standard: D638-10
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Knight, T.A., Payne, R.G. (2013). Characterization of a PGA-Based Scaffold for Use in a Tissue-Engineered Neo-Urinary Conduit. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_15
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DOI: https://doi.org/10.1007/978-1-62703-363-3_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-362-6
Online ISBN: 978-1-62703-363-3
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