Abstract
The following chapter outlines methodologies to phenotypically characterize primary cells for the use in tissue-engineered and regenerative medicine applications. Methods covered include analyzing cells using immunocytochemistry, fluorescence-activated cell sorting, and confocal microscopy of adherent and suspended cells, as well as combinations of formulated cell–biomaterial constructs.
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Acknowledgments
The authors acknowledge Tengion Inc., where all work was carried out. The analysis of the cell–biomaterial combinations would not have been possible without help of the Biomaterials Development group at Tengion, who provided testing material.
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Burnette, T.B., Bruce, A.T. (2013). Phenotypic Analysis of Bioactive Cells for Application in Regenerative Medicine. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_10
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DOI: https://doi.org/10.1007/978-1-62703-363-3_10
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