Abstract
Glycoproteins play an important role in cell signaling and cell–cell interaction. The alterations of glycoproteins are often relevant to progression of diseases, and these changed glycoproteins can be important biomarkers. The lectin-based glycoproteomic technology has extensively been used for high-throughput screening of potential glycoprotein biomarkers. Here we describe a multi-lectin affinity chromatography and label-free quantitative glycoproteomic approach for discovery of glycoprotein biomarkers relevant to differentiation of glioblastoma stem cells.
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Acknowledgments
This work was funded under the National Institute of Health Grant No. R01 49500 (D.M.L.) and the National Cancer Institute Grant R21 CA124441 (D.M.L.) and R01 CA154455 (D.M.L.).
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He, J., Liu, Y., Wu, J., Lubman, D.M. (2013). Analysis of Glycoproteins for Biomarker Discovery. In: Zhou, M., Veenstra, T. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology, vol 1002. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-360-2_10
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DOI: https://doi.org/10.1007/978-1-62703-360-2_10
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Publisher Name: Humana Press, Totowa, NJ
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