Molecular Detection of t(14;18)(q32;q21) in Follicular Lymphoma

  • C. Cameron Yin
  • Rajyalakshmi Luthra
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 999)

Abstract

The t(14;18)(q32;q21) can be detected in approximately 80% of cases of follicular lymphoma (FL). This translocation juxtaposes the BCL2 oncogene at 18q21 with the IGH@ at 14q32, and leads to overexpression of BCL2 protein which protects the cells from apoptosis. The high degree of sequence homology among the 3′ portion of the JH segments and the clustering of breakpoints on chromosome 18 make the IGH@/BCL2 very amenable to polymerase chain reaction (PCR) detection. We describe two multiplex TaqMan-based real-time PCR assays which can be used to detect and quantify the major and minor breatpoint cluster regions of IGH@/BCL2 fusion products in newly diagnosed FL, and to monitor minimal residual disease during treatment or early relapse.

Key words

Follicular lymphoma t(14;18)(q32;q21) PCR 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • C. Cameron Yin
    • 1
  • Rajyalakshmi Luthra
    • 1
  1. 1.Department of HematopathologyThe University of Texas MD Anderson Cancer CenterHoustonUSA

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