Detection of Genetic Translocations in Lymphoma Using Fluorescence In Situ Hybridization

  • Kaaren K. Reichard
  • Sheldon Robinett
Part of the Methods in Molecular Biology book series (MIMB, volume 999)


Certain lymphoma types are characterized by recurring genetic translocations. Detection of these translocations enables confirmation of a suspected diagnosis and provides a genetic marker which can be subsequently monitored and followed. Rapid and reliable identification of these molecular rearrangements is a key component in the workup of lymphoma. While conventional cytogenetics may be a useful tool in this regard, fluorescence in situ hybridization (FISH) offers additional advantages including the ability to use formalin-fixed tissues, no requirement for dividing cells, ability to score many cells, improved sensitivity, and faster turnaround time for results.

Key words

Lymphoma Translocation FISH 


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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Kaaren K. Reichard
    • 1
  • Sheldon Robinett
    • 2
  1. 1.Department of Laboratory Medicine and Pathology, Division of HematopathologyMayo Clinic RochesterRochesterUSA
  2. 2.Department of PathologyUniversity of New MexicoAlbuquerqueUSA

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