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Detection of Genetic Translocations in Lymphoma Using Fluorescence In Situ Hybridization

  • Kaaren K. Reichard
  • Sheldon Robinett
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 999)

Abstract

Certain lymphoma types are characterized by recurring genetic translocations. Detection of these translocations enables confirmation of a suspected diagnosis and provides a genetic marker which can be subsequently monitored and followed. Rapid and reliable identification of these molecular rearrangements is a key component in the workup of lymphoma. While conventional cytogenetics may be a useful tool in this regard, fluorescence in situ hybridization (FISH) offers additional advantages including the ability to use formalin-fixed tissues, no requirement for dividing cells, ability to score many cells, improved sensitivity, and faster turnaround time for results.

Key words

Lymphoma Translocation FISH 

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Copyright information

© Springer Science+Business Media, New York 2013

Authors and Affiliations

  • Kaaren K. Reichard
    • 1
  • Sheldon Robinett
    • 2
  1. 1.Department of Laboratory Medicine and Pathology, Division of HematopathologyMayo Clinic RochesterRochesterUSA
  2. 2.Department of PathologyUniversity of New MexicoAlbuquerqueUSA

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