Abstract
There has been long-standing interest in generating fibers from structural proteins and a great deal of work has been done in attempting to mimic dragline spider silk. Dragline silk balances stiffness, strength, extensibility, and high energy to break. Mimicking these properties through aqueous-based spinning of recombinant silk protein is a significant challenge; however, an approach has been developed that facilitates the formation of fibers approaching the mechanical properties seen with natural dragline silk. Due to the multitude of solution, spinning and post-spinning variables one has to consider, the method entails a multivariate approach to protein solution processing and fiber spinning. Optimization to maximize mechanical integrity of the fibers is performed by correlating the solution and spinning variables to mechanical properties and using this information for subsequent fiber spinning studies. Here, the method is described in detail and emphasizes the lessons learned during the iterative variable analysis process, which can be used as a basis for aqueous-based fiber spinning of other structural proteins.
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References
Vollrath F, Knight DP (2001) Liquid crystalline spinning of spider silk. Nature 410:541–548
Vollrath F, Madsen B, Shao ZZ (2001) The effect of spinning conditions on the mechanics of a spider’s dragline silk. Proc R Soc Lond B Biol Sci 268:2339–2346
Shao ZZ, Vollrath F (2002) Materials: Surprising strength of silkworm silk. Nature 418:741–741
Jin HJ, Kaplan DL (2003) Mechanism of silk processing in insects and spiders. Nature 424:1057–1061
Seidel A, Liivak O, Jelinski LW (1998) Artificial spinning of spider silk. Macromolecules 31:6733–6736
Seidel A, Liivak O, Calve S, Adaska J, Ji GD, Yang ZT, Grubb D, Zax DB, Jelinski LW (2000) Regenerated spider silk: Processing, properties, and structure. Macromolecules 33:775–780
Trabbic KA, Yager P (1998) Comparative structural characterization of naturally- and synthetically-spun fibers of Bombyx mori fibroin. Macromolecules 31:462–471
Liivak O, Blye A, Shah N, Jelinski LW (1998) A microfabricated wet-spinning apparatus to spin fibers of silk proteins. Structure-property correlations. Macromolecules 31:2947–2951
Lazaris A, Arcidiacono S, Huang Y, Zhou JF, Duguay F, Chretien N, Welsh EA, Soares JW, Karatzas CN (2002) Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science 295:472–476
Arcidiacono S, Mello CM, Butler M, Welsh E, Soares JW, Allen A, Ziegler D, Laue T, Chase S (2002) Aqueous processing and fiber spinning of recombinant spider silks. Macromolecules 35:1262–1266
Fahnestock S (2001) Recombinantly produced spider silk, 31 Jul 2001. US patent 6,628,169
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Arcidiacono, S., Welsh, E.A., Soares, J.W. (2013). Aqueous-Based Spinning of Fibers from Self-Assembling Structural Proteins. In: Gerrard, J. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 996. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-354-1_3
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DOI: https://doi.org/10.1007/978-1-62703-354-1_3
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