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Ion Channels pp 3–19Cite as

Approaches to Cloning of Pain-Related Ion Channel Genes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 998))

Abstract

Molecular pain research is a relatively new and rapidly expanding field that represents advancement in conventional pain research. One of the fundamentals of molecular pain involves the cloning of genes and especially the ion channels specifically involved in nociceptive processing at the periphery and centrally. A variety of approaches were used to isolate these critically important genes. Cloning of these genes involved innovative strategies based on existing molecular approaches. This review will discuss well-utilized cloning approaches and their exploitation in molecular pain research.

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Acknowledgements

I would like to thank to members of my lab Dr. Belugin, Mr. Patil, Ms. Salas, and Ms. Phoebe as well as collaborators Drs. Wood, Hargreaves, Henry, Jeske, Diogenes, Gamper Staruschenko, Brooks, Dube, Nikita Ruparel, Shivani Ruparel, and Patwardhan for helping me in contribution to molecular pain research. Supporting grants are DE014928 and DE019311.

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Authors and Affiliations

  1. Department of Endodontics, Dental School, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA

    Armen N. Akopian

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  1. Armen N. Akopian
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  1. School of Biomedical Sciences, University of Leeds, Garstang Bld. 6.31d, Leeds, LS2 9JT, United Kingdom

    Nikita Gamper

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Akopian, A.N. (2013). Approaches to Cloning of Pain-Related Ion Channel Genes. In: Gamper, N. (eds) Ion Channels. Methods in Molecular Biology, vol 998. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-351-0_1

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  • DOI: https://doi.org/10.1007/978-1-62703-351-0_1

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