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Methods for Culturing Human Embryonic Stem Cells on Feeders

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Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 997))

Abstract

Human embryonic stem cells can be cultured and maintained on fibroblast feeder cells of murine or human origin. Thorough protocols are provided for the growth and maintenance of human embryonic stem cells on either feeder cell type.

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References

  1. Evans MJ, Kaufman MH (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292:154–156

    Article  PubMed  CAS  Google Scholar 

  2. Martin GR (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 78:7634–7638

    Article  PubMed  CAS  Google Scholar 

  3. Robertson EJ (ed) (1987) Teratocarcinomas and embryonic stem cells: a practical approach. IRL, Oxford

    Google Scholar 

  4. Thomas KR, Capecchi MR (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51:503–512

    Article  PubMed  CAS  Google Scholar 

  5. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145–1147

    Article  PubMed  CAS  Google Scholar 

  6. Richards M, Fong CY, Chan WK et al (2002) Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nat Biotechnol 20:933–936

    Article  PubMed  CAS  Google Scholar 

  7. Lin S, Talbot P (2011) Methods for culturing mouse and human embryonic stem cells. Methods Mol Biol 690:31–56

    Article  PubMed  CAS  Google Scholar 

  8. Eiselleova L, Peterkova I, Neradil J, Slaninova I, Hampl A, Dvorak P (2008) Comparative study of mouse and human feeder cells for human embryonic stem cells. Int J Dev Biol 52(4):353–363. doi:10.1387/ijdb.082590le

    Article  PubMed  CAS  Google Scholar 

  9. Cote RJ (2001) Aseptic technique for cell culture. Curr Protoc Cell Biol Chapter 1: Unit 1.3

    Google Scholar 

  10. Phelan MC (2006) Techniques for mammalian cell tissue culture. Curr Protoc Hum Genet Appendix 3: Appendix 3G

    Google Scholar 

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Author information

Authors and Affiliations

  1. Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA

    Jasmeet Kaur

  2. Primary and Stem Cell Systems, Life Technologies, Frederick, MD, USA

    Mary Lynn Tilkins

Authors
  1. Jasmeet Kaur
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  2. Mary Lynn Tilkins
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Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Van Allen Way 5781, Carlsbad, 92008, California, USA

    Uma Lakshmipathy

  2. , Primary and Stem Cell Systems, Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mohan C Vemuri

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© 2013 Springer Science+Business Media New York

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Kaur, J., Tilkins, M.L. (2013). Methods for Culturing Human Embryonic Stem Cells on Feeders. In: Lakshmipathy, U., Vemuri, M. (eds) Pluripotent Stem Cells. Methods in Molecular Biology, vol 997. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-348-0_8

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  • DOI: https://doi.org/10.1007/978-1-62703-348-0_8

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-347-3

  • Online ISBN: 978-1-62703-348-0

  • eBook Packages: Springer Protocols

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