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Pluripotent Stem Cells pp 57–72Cite as

Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essential 8™ Medium Conditions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 997))

Abstract

Human-induced pluripotent stem cells (iPSCs) are an important potential source of cells for regenerative medicine due to their inherent ability to differentiate into all cell types of the three germ layers. Generation of iPSCs with a non-integrating reprogramming method and in culture conditions that are completely absent of animal proteins will be ideal for such regenerative and cell therapy applications. Here we describe a method to generate non-integrating iPSCs using the Episomal iPSC Reprogramming Vectors.

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References

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Author information

Authors and Affiliations

  1. Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA

    Andrew Fontes, Chad C. MacArthur & Pauline T. Lieu

  2. Primary and Stem Cell Systems, Life Technologies, Frederick, MD, USA

    Mohan C. Vemuri

Authors
  1. Andrew Fontes
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  2. Chad C. MacArthur
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  3. Pauline T. Lieu
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  4. Mohan C. Vemuri
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Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Van Allen Way 5781, Carlsbad, 92008, California, USA

    Uma Lakshmipathy

  2. , Primary and Stem Cell Systems, Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mohan C Vemuri

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© 2013 Springer Science+Business Media New York

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Fontes, A., MacArthur, C.C., Lieu, P.T., Vemuri, M.C. (2013). Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essential 8™ Medium Conditions. In: Lakshmipathy, U., Vemuri, M. (eds) Pluripotent Stem Cells. Methods in Molecular Biology, vol 997. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-348-0_6

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  • DOI: https://doi.org/10.1007/978-1-62703-348-0_6

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-347-3

  • Online ISBN: 978-1-62703-348-0

  • eBook Packages: Springer Protocols

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