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Pluripotent Stem Cells pp 35–43Cite as

Generation of Human-Induced Pluripotent Stem Cells by Lentiviral Transduction

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 997))

Abstract

Human somatic cells can be reprogrammed to the pluripotent state to become human-induced pluripotent stem cells (hiPSC). This reprogramming is achieved by activating signaling pathways that are expressed during early development. These pathways can be induced by ectopic expression of four transcription factors—Oct4, Sox2, Klf4, and c-Myc. Although there are many ways to deliver these transcription factors into the somatic cells, this chapter will provide protocols that can be used to generate hiPSC from lentiviruses.

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Acknowledgment

Supported by NIH U24 MH068457, RC1 CA147187, and R21 DA032984-01.

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Authors and Affiliations

  1. Rutgers NIMH Stem Cell Center and Department of Genetics, Rutgers, The State University of New Jersey, Nelson Biological Laboratory, Piscataway, NJ, USA

    Jennifer C. Moore

Authors
  1. Jennifer C. Moore
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Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Van Allen Way 5781, Carlsbad, 92008, California, USA

    Uma Lakshmipathy

  2. , Primary and Stem Cell Systems, Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mohan C Vemuri

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Moore, J.C. (2013). Generation of Human-Induced Pluripotent Stem Cells by Lentiviral Transduction. In: Lakshmipathy, U., Vemuri, M. (eds) Pluripotent Stem Cells. Methods in Molecular Biology, vol 997. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-348-0_4

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  • DOI: https://doi.org/10.1007/978-1-62703-348-0_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-347-3

  • Online ISBN: 978-1-62703-348-0

  • eBook Packages: Springer Protocols

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