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A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells

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Pluripotent Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 997))

Abstract

Here, we present a highly efficient and reproducible method for the establishment of mouse embryonic stem cells (mESCs) from embryonic day 3.5 (E3.5) whole blastocysts. This protocol involves the use of small molecules SB431542 and PD0325901, which inhibit transforming growth factor-β (TGF-β) and extracellular signal-regulated kinases (ERK1/2), respectively. This protocol is universal in the derivation of mESC lines from NMRI, C57BL/6, BALB/c, DBA/2, and FVB/N strains, which have previously been considered refractory or non-permissive for ESC establishment. The efficiency of mESC lines generation is 100%, regardless of genetic background.

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Acknowledgments

This study was funded by grants provided from Royan Institute, the Iranian Council of Stem Cell Technology, and the Iran National Science Foundation (INSF).

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Authors and Affiliations

  1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Hossein Baharvand & Seyedeh-Nafiseh Hassani

  2. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran

    Hossein Baharvand & Seyedeh-Nafiseh Hassani

Authors
  1. Hossein Baharvand
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  2. Seyedeh-Nafiseh Hassani
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Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Van Allen Way 5781, Carlsbad, 92008, California, USA

    Uma Lakshmipathy

  2. , Primary and Stem Cell Systems, Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mohan C Vemuri

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Baharvand, H., Hassani, SN. (2013). A New Chemical Approach to the Efficient Generation of Mouse Embryonic Stem Cells. In: Lakshmipathy, U., Vemuri, M. (eds) Pluripotent Stem Cells. Methods in Molecular Biology, vol 997. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-348-0_2

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  • DOI: https://doi.org/10.1007/978-1-62703-348-0_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-347-3

  • Online ISBN: 978-1-62703-348-0

  • eBook Packages: Springer Protocols

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