Abstract
Mis-regulation of gene expression due to epigenetic abnormalities has been linked with complex genetic disorders, psychiatric illness, and cancer. In addition, the dynamic epigenetic changes that occur in pluripotent stem cells are believed to impact regulatory networks essential for proper lineage development. Chromatin immunoprecipitation (ChIP) is a technique used to enrich genomic fragments using antibodies against specific chromatin modifications, such as DNA-binding proteins or modified histones. Until recently, many ChIP protocols required large numbers of cells for each immunoprecipitation. This severely limited analysis of rare cell populations or post-mitotic, differentiated cell lines. Here, we describe a low cell number ChIP protocol with next generation sequencing and analysis that has the potential to uncover novel epigenetic regulatory pathways that were previously difficult or impossible to obtain.
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Acknowledgements
This work is supported by grants from NIH (1R21MH085088 and 1RC1CA147187). C.L.R. was an NSF IGERT fellow (DGE 0801620).
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Ricupero, C.L., Swerdel, M.R., Hart, R.P. (2013). Epigenome Analysis of Pluripotent Stem Cells. In: Lakshmipathy, U., Vemuri, M. (eds) Pluripotent Stem Cells. Methods in Molecular Biology, vol 997. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-348-0_16
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DOI: https://doi.org/10.1007/978-1-62703-348-0_16
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