Abstract
Nanoparticle-based drug delivery systems have considerable potential for improvement of drug stability, bioavailability, and reduced dosing frequency. Important technological advantages of nanoparticles include high carrier capacity across biological membranes and controlled drug release. Ultimately, success of nanodelivery systems depends on toxicologic issues associated with the understanding of the fate of nanocarriers and their polymeric constituents within the targeted cells. Here we describe a method for determining subcellular distribution of nanoparticles by isolation and identification of organelles that come in direct contact with these structures.
Equal contributions were made by Ari Nowacek and Irena Kadiu.
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References
Nowacek A, Gendelman HE (2009) NanoART, neuroAIDS and CNS drug delivery. Nanomedicine (Lond) 4:557–574
Nowacek A, Kosloski LM, Gendelman HE (2009) Neurodegenerative disorders and nanoformulated drug development. Nanomedicine (Lond) 4:541–555
Kadiu I, Nowacek A, McMillan J, Gendelman HE (2011) Macrophage endocytic trafficking of antiretroviral nanoparticles. Nanomedicine 6:25–42
Acknowledgments
The work was supported by the National Institutes of Health grants 1P01 DA028555, 2R01 NS034239, 2R37 NS36126, P01 NS31492, P20RR 15635, P01MH64570, and P01 NS43985 (to H.E.G.) and from a research grant from Baxter Healthcare. The authors thank Ms. Robin Taylor for critical reading of the manuscript and outstanding graphic and literary support.
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Nowacek, A., Kadiu, I., McMillan, J., Gendelman, H.E. (2013). Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments. In: Weissig, V., Elbayoumi, T., Olsen, M. (eds) Cellular and Subcellular Nanotechnology. Methods in Molecular Biology, vol 991. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-336-7_6
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DOI: https://doi.org/10.1007/978-1-62703-336-7_6
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-335-0
Online ISBN: 978-1-62703-336-7
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